Table I Proteins differentially expressed in mouse cerebellum upon Purkinje cell DNA damage
UniprotKB accession no.Protein nameDescription8w FCa16w FCa26w FCaq value
Classical Purkinje cell markers
    P12658Spot 35/CalbindinCalbindin−0.1−0.9−2.40.000
    P12660L7/Pcp2Purkinje cell protein 2−0.1−1.1−2.60.000
    P63054PEP-19/Pcp4Purkinje cell protein 40.0−0.8−1.60.000
Transmembrane proteins
    P97772mGluR1Metabotropic glutamate receptor 1−0.1−1.0−2.00.000
    Q61625GluRδ2Glutamate receptor δ-2 subunit−0.2−1.5−2.40.000
    P23818GluR-1Glutamate receptor 1, AMPA10.3−0.4−1.10.027
    Q9WV18GABABR1γ-Aminobutyric acid type B receptor subunit 1−0.1−0.6−1.40.027
    Q80T41GABABR2γ-Aminobutyric acid type B receptor subunit 2−0.1−0.8−1.30.017
    P11881IP3R1Inositol 1,4,5-trisphosphate receptor type 1−0.1−1.1−2.60.000
    Q9Z329IP3R2Inositol 1,4,5-trisphosphate receptor type 20.0−0.9−2.60.000
    O55143SERCA2Sarcoplasmic/endoplasmic reticulum calcium ATPase 20.0−0.5−1.00.016
    Q64518SERCA3Sarcoplasmic/endoplasmic reticulum calcium ATPase 3−0.1−1.0−2.80.000
    Q9QZC1TrpC3Short transient receptor potential channel 30.1−0.9−4.50.019
    P63143Kvβ1Voltage-gated potassium channel subunit β-1−0.1−0.7−1.20.016
    Q6PHS9Cavα2δ2Voltage-gated calcium channel subunit δ-2−0.3−0.8−1.20.017
    O35544Eaat4Excitatory amino acid transporter 4−0.2−0.9−1.90.000
    Q5DTL9Slc4a10Sodium-driven chloride bicarbonate exchanger−0.2−0.6−1.20.050
    Q921R8Slc41a3Solute carrier family 41 member 30.2−0.9−1.40.084
    Q6WQJ1DGL-αSn1-specific diacylglycerol lipase α−0.1−1.1−2.5ND*
    Q8JZM4DNERδ and Notch-like epidermal growth factor-related receptor−0.2−0.9−1.50.000
    Q8C5W0CalminCalmin/Calponin-like transmembrane domain protein0.0−1.0−1.70.000
    Q6NXK7DPP XDipeptidyl peptidase X−0.1−0.6−1.50.000
    Q62092Nsg1Neuron-specific protein family member 1−0.3−0.5−1.20.017
    Q3UH99Shisa6UPF0626 protein B−0.2−1.0−1.10.081
    Q8K0T0Rtn1Reticulon-10.0−0.5−1.00.061
    P41731Cd63CD63 antigen0.10.41.10.082
    P41233ABC-1ATP-binding cassette subfamily A member 1ND*0.51.20.082
    Q08481PECAM-1Platelet endothelial cell adhesion molecule (CD31)ND*ND*1.10.082
    Q8R2Y2MCAMCell surface glycoprotein MUC18/melanoma cell adhesion molecule/CD1460.40.81.40.082
    P23242Cx43Gap junction α-1 protein/Connexin-430.40.51.30.082
Extracellular proteins
    Q80YX1TN-CTenascin-C/Hexabrachion0.0−1.6−3.20.000
    P02802MT-1Metallothionein-1−0.10.51.10.082
    P03995GFAPGlial fibrillary acidic protein0.01.72.60.082
    Q9R118HTRA1Serine protease HTRA1−0.10.31.90.082
    Q60675Lama2Laminin subunit α-2/laminin M0.70.62.00.082
Scaffold proteins
    Q0QWG9DelphilinDelphilin/Glutamate receptor, ionotropic, δ2-interacting protein 1−0.1−1.3−3.10.000
    Q6WVG3Kctd12BTB/POZ domain-containing protein KCTD120.0−0.6−1.10.017
    Q80Z38Shank2SH3 and multiple ankyrin repeat domains protein 20.0−1.1−2.00.029
    Q99JP6Homer-3Homer protein homolog 3−0.1−1.1−2.50.000
    O54931Akap2A-kinase anchor protein 2−0.2−0.7−1.40.000
Signaling proteins
    P11798CaMK-IIαCalcium/calmodulin-dependent protein kinase type II α chain0.0−0.9−1.70.071
    P63318PKCγProtein kinase C γ type−0.1−1.3−2.50.000
    P0C605cGK1cGMP-dependent protein kinase 1, α isozyme−0.4−2.5−3.3ND*
    Q8CG03Pde5acGMP-specific 3′,5′-cyclic phosphodiesterase−0.4−1.0−2.70.000
    Q8R071IP3KAInositol-trisphosphate 3-kinase A0.0−1.1−3.00.000
    Q9EPW0Inpp4aType I inositol-3,4-bisphosphate 4-phosphatase0.0−0.6−1.30.026
    Q91WG7DGK-γDiacylglycerol kinase γ0.0−0.6−1.02.957
    Q80UP3DGK-ζDiacylglycerol kinase ζ0.1−0.9−1.70.079
    Q8BW86Arhgef33Rho guanine nucleotide exchange factor 330.0−1.3−2.90.000
    Q8BXT1RGS8Regulator of G-protein signaling 8−0.1−1.3−2.90.000
    Q9JMF3GNG13Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit γ-13−0.1−1.1−2.10.016
    P57759Erp29Endoplasmic reticulum protein ERp290.0−0.6−1.40.000
    P6851014-3-3η/Ywhah14-3-3 protein η−0.1−0.4−1.00.050
Cytosekeletal proteins
    Q9JJZ2Tuba8Tubulin α-8 chain−0.2−0.8−1.80.000
    P97434RIP3Myosin phosphatase Rho-interacting protein−0.3−0.7−1.60.000
    Q05BC3EMAP-1Echinoderm microtubule-associated protein-like 10.0−0.4−1.30.000
    Q3UJU9RMD-3Regulator of microtubule dynamics protein 30.1−0.6−1.60.000
    Q8R1S4Mtss1Metastasis suppressor protein 1−0.1−0.9−2.40.000
Cytosolic and remaining proteins
    P28651CARPCarbonic anhydrase-related protein−0.1−1.1−2.60.000
    Q3TGF2Fam107bProtein FAM107B−0.1−1.1−2.00.000
    Q99K30EPS8-LP2Epidermal growth factor receptor kinase substrate 8-like protein 2−0.1−1.1−2.50.000
    Q9ERG2Striatin-3Striatin-3/Cell cycle autoantigen SG2NA0.2−0.7−1.60.000
    P62748Hpcal1Hippocalcin-like protein 1/Neural visinin-like protein 30.0−0.7−1.30.017
    Q61704ITI-HC3Inter-α-trypsin inhibitor heavy chain H30.1−0.6−1.70.020
    Q9ERQ8CA-VIICarbonic anhydrase 70.2−0.8−1.40.028
    Q8BKX1BAI-ap2Brain-specific angiogenesis inhibitor 1-associated protein 2/Insulin receptor substrate p53−0.1−0.8−1.20.030
    Q1RLL3Copine-9Copine-9−0.1−0.7−1.40.081
    Q9CRD4Dbndd2Dysbindin domain-containing protein 20.2−0.9−1.50.082
    Q80ZW2Them6UPF0670 protein THEM6 homolog0.00.0−1.10.084
    Q3UVC0Ksr2Kinase suppressor of Ras 2−0.4−0.9−1.10.086
    Q8BIZ1AIDA-1Amyloid-β protein intracellular domain-associated protein 10.0−0.7−1.30.097
    P28828R-PTPμReceptor-type tyrosine-protein phosphatase mu0.2−0.2−1.10.078
    Q02105C1qcComplement C1q subcomponent subunit CND*ND*2.00.082
    P20152VimVimentin0.10.21.10.082
    P20060Hexbβ-Hexosaminidase subunit β0.40.71.20.082
    O70370CtssCathepsin S0.30.81.20.082
    Q06890Clu /ApoJClusterin/Apolipoprotein J0.01.01.40.082
    Q8BVW0GancNeutral α-glucosidase C−0.20.11.00.082
    Q08642Padi2Protein-arginine deiminase type-20.00.81.30.082
    Q8VDD9PHIPPH-interacting protein/Neuronal differentiation-related protein0.0−0.71.00.082
    Q8BVW0GancNeutral α-glucosidase C−0.20.11.00.082
    Q9R0M4PCLP-1Podocalyxin-like protein 1−1.20.31.20.082
    Q8VHQ3Ppp1r16bProtein phosphatase 1 regulatory inhibitor subunit 16B−0.1−1.4ND*ND*
    P16110Gal-3/Mac-2Galectin-3, Mac-2 antigenND*1.63.00.081
    P23927Cryabα-Crystallin B chain−0.10.41.10.082
    P42574CASP-3Caspase 30.6ND3.8ND*
  • a FC indicates log2 fold change of protein expression between control and KO cerebella after 8 weeks (8w), 16 weeks (16w), and 26 weeks (26w) of age.

  • * means not quantified in one or two replicates due to absence in KO or control tissue (possibly an on/off-regulation). q values were calculated for protein ratios from 26-week-old mice.