Table II Comparison of the enriched (p < 0.01) proteins of the β-tubulin ICPL-IP versus SILAC-IP from HEK293T cells. Ratios are the mean of three biological replicates
ICPL-IPSILAC-IPUniprot IDGene NameProtein NamesTubulin/Interactor
Ratio β-tubulin/control NormalizedRatio β-tubulin/control Normalized Significance ARatio β-tubulin/control NormalizedRatio β-tubulin/control Normalized Significance A
56.512.14E-068.555.93E-04Q00587-1BORG5Binder of Rho GTPases 5yes
46.296.22E-065.764.39E-03Q9BVA1TUBB2BTubulin beta-2B chainyes
43.838.26E-0618.863.57E-06P07437TUBBTubulin beta chain;Tubulin beta-5 chainyes
36.512.07E-0522.728.64E-07Q3ZCM7TUBB8Tubulin beta-8 chainyes
31.404.29E-0513.294.13E-05P31689DNAJ2DnaJ homolog subfamily A member 1yes
24.531.33E-049.044.33E-04Q96EY1-1DNAJA3DnaJ homolog subfamily A member 3yes
17.945.02E-047.251.43E-03P42677MPS140S ribosomal protein S27yes
17.815.17E-0416.678.74E-06P68371TUBB2CTubulin beta-2 chain;Tubulin beta-2C chainyes
15.209.65E-0412.336.69E-05Q01726MC1RMelanocortin receptor 1yes
14.181.26E-0312.585.88E-05O60884CPR3Cell cycle progression restoration gene 3 proteinyes
12.971.75E-036.163.21E-03Q9BSD7C1orf57Nucleoside triphosphate phosphohydrolaseyes
10.383.84E-03--O95831-1AIFApoptosis-inducing factor 1yes
10.174.11E-034.839.60E-03P25705ATP5AATP synthase subunit alphayes
9.814.64E-03--O14654IRS4160 kDa phosphotyrosine protein; Insulin receptor substrate 4yes
9.764.72E-036.472.53E-03Q13885TUBB2Tubulin beta-2A chainyes
9.155.83E-038.844.91E-04P68363TUBA1BAlpha-tubulin ubiquitous;Tubulin alpha-1B chainyes
7.749.87E-03--Q9BRX2CGI-17Protein pelota homologyes