Table I Proteins differentially secreted by KM12SM and KM12C CRC cells with at least 2-fold change variation

MS/MS spectra for those proteins with single peptide entries are shown in supplemental Fig. S2.

DescriptionAbreviationAccession no.Forward SILAC (heavy KM12SM vs. light KM12C)Reverse SILAC (light KM12SM vs. heavy KM12C)
-Fold changeaMascot scoreCoverageNo. of peptides-Fold changeaMascot scoreCoverageNo. of peptides
Protein S100-A8S100A8P05109>30833.4153.768>30181.247.315
Tumor necrosis factor ligand superfamily member 9CD137L/TNFSF9P4127327.73397.231.506396.691
Podocalyxin-like protein 1PODXLO0059210.893897.35419.3415810.576
MidkineMDKP2174112.61506.991-306.991
Cathepsin SCTSSP2577415.1520213.2942.7710517.825
Glutathione S-transferase PGSTP1P092116.92272863.33910.79166057.148
Neurosecretory protein VGFVGFO152409.662998.9467.2263126.8314
Carcinoembryonic antigen-related cell adhesion molecule 5CEACAM5P067318.345987.5545.9368512.547
Growth/differentiation factor 15GDF15Q999886.21309638.96115.81249345.7812
Zymogen granule protein 16 homolog BZG16BQ96DA03.4355729.8156.4019729.815
Selenium-binding protein 1SELENBP1Q132283.7013813.1454.49339.324
General transcription factor IIF subunit 1GTF2F1P352694.90433.0913.17443.091
General transcription factor II-IGTF2IP783473.03733.6134.07461.701
Sclerostin domain-containing protein 1SOSTDC1Q6X4U43.375113.593----
Protein ETHE1, mitochondrialETHE1O955712.226726.7764.163326.776
Brain-specific serine protease 4PRSS22Q9GZN44.3044824.6161.6117616.095
Putative maltase-glucoamylase-like protein FLJ16351MGAL1Q6ZN802.60422.9423.0614011.766
FascinFSCN1Q166582.9113818.2692.647910.555
Proteasome subunit β type-9PSMB9P280653.14738.6822.16894.571
Proteasome subunit β type-8PSMB8P280622.2346519.9352.9721425.007
Src homology 3 domain-containing kinase-binding protein 1SH3KBP1Q96B972.49571.6512.66221.651
MelanotransferrinMFI2P085821.92307.3263.211404.073
YTH domain family protein 2YTHDF2Q9Y5A91.9810012.6182.98308.815
V-type proton ATPase subunit B, brain isoformATP6V1B2P212811.7414216.4473.19347.243
Endothelial protein C receptorPROCRQ9UNN82.8168214.7132.07119326.055
Ribosyldihydronicotinamide dehydrogenase (quinone)NQO2P160831.3010119.0532.975711.262
Acyl-protein thioesterase 1LYPLA1O756081.5713320.0042.504813.913
Activated RNA polymerase II transcriptional co-activator p15SUB1P539990.499718.9020.536918.902
Adenylate kinase isoenzyme 1AK1P005680.527517.0130.4815328.355
10-kDa heat shock protein, mitochondrialHSPE1P616040.5086765.6980.5058157.847
39 S ribosomal protein L12, mitochondrialMRPL12P528150.5311622.7350.475718.184
3-Hydroxyisobutyrate dehydrogenase, mitochondrialHIBADHP319370.46293.8710.541038.632
Complement decay-accelerating factorCD55P081740.5151624.1590.4739321.788
tRNA (adenine-N(1)-)-methyltransferase noncatalytic subunit TRM6TRMT6Q9UJA50.52213.8210.45343.821
Synaptogyrin-2SYNGR2O437600.473620.5450.494112.503
Toll-interacting proteinTOLLIPQ9H0E20.536311.6840.3923224.456
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-α-mannosidaseMAN1B1Q9UKM70.44272.7220.47246.724
78-kDa glucose-regulated proteinHSPA5P110210.41245543.43260.49487421.1011
Solute carrier family 12 member 2SLC12A2P550110.492166.7750.401054.133
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit γ12GNG12Q9UBI60.484837.5020.4118237.502
Serine/threonine-protein phosphatase PGAM5, mitochondrialPGAM5Q96HS10.47317.2720.404714.884
Tax1-binding protein 3TAX1BP3O149070.4417725.0020.4110925.002
Tetraspanin-8TSPAN8P190750.50454.2210.35997.592
Stomatin-like protein 2STOML2Q9UJZ10.4855831.1880.3775225.287
Transmembrane emp24 domain-containing protein 9TMED9Q9BVK60.426221.2840.421708.512
Transcription intermediary factor 1-βTRIM28Q132630.43526.1150.411348.865
Transmembrane emp24 domain-containing protein 7TMED7Q9Y3B30.442911.1620.395214.733
α-N-acetylgalactosaminide α-2,6-sialyltransferase 1ST6GALNAC1Q9NSC70.43252.8320.40704.332
MARCKS-related proteinMARCKSL1P490060.5014914.3620.3255014.362
Ras-related protein Rab-25RAB25P577350.464813.1530.3529736.627
Translocon-associated protein subunit δSSR4P515710.4922036.4250.3224936.425
Cytochrome c oxidase subunit 5A, mitochondrialCOX5AP206740.496216.0020.328116.673
Hydroxymethylglutaryl-CoA synthase, cytoplasmicHMGCS1Q015810.42392.5010.38352.501
ADP-ribosylation factor 6ARF6P623300.5127326.8640.2946538.865
Cytochrome b-c1 complex subunit Rieske, mitochondrialUQCRFS1P479850.46308.0320.3314418.614
NephronectinNPNTQ6UXI90.42507.7940.3711310.445
Prohibitin-2PHB2Q996230.4659239.13110.33135946.8213
CD59 glycoproteinCD59P139870.519415.6330.2614025.003
Electron transfer flavoprotein subunit α, mitochondrialETFAP138040.4218227.6370.3495643.2411
Ras-related protein Rap-2cRAP2CQ9Y3L50.532216.3930.24326.011
RNA-binding protein RalyRALYQ9UKM90.444012.7540.328326.477
ATP synthase subunit δ, mitochondrialATP5DP300490.4424813.6920.3212613.692
Sodium/potassium-transporting ATPase subunit β-1ATP1B1P050260.50108528.7180.2599028.718
Transmembrane emp24 domain-containing protein 4TMED4Q7Z7H50.506612.3330.24458.812
Laminin subunit α-5LAMA5O152300.482943.25100.261261.384
Cytochrome b-c1 complex subunit 2, mitochondrialUQCRC2P226950.49703.5310.2530932.2310
Syndecan-1SDC1P188270.4841716.1350.244595.481
Nucleotide exchange factor SIL1SIL1Q9H1730.4518013.8860.27677.383
Voltage-dependent anion-selective channel protein 1VDAC1P217960.4151730.0470.30168165.7215
Neutral α-glucosidase ABGANABQ146970.44101427.75240.27362520.5520
ADP-ribosylation factor 4ARF4P180850.5117417.2230.1935630.005
Cytochrome c oxidase subunit 4 isoform 1, mitochondrialCOX4I1P130730.4313518.3430.2615518.343
Peroxiredoxin-4PRDX4Q131620.5132819.9360.1427615.874
Cathepsin DCTSDP073390.321143052.43210.251344355.3425
ATP synthase subunit α, mitochondrialATP5A1P257050.3526518.6390.23101736.8918
Stromal cell-derived factor 2-like protein 1SDF2L1Q9HCN80.305019.9130.26495.881
AgrinAGRNO004680.3314589.34160.2125569.1916
Ephrin type-A receptor 4EPHA4P547640.37422.6430.161195.484
Peptidyl-prolyl cis-trans isomerase CPPICP458770.3045116.5140.1869817.454
Trifunctional enzyme subunit β, mitochondrialHADHBP550840.35265.9130.13765.703
45-kDa calcium-binding proteinSDF4Q9BRK50.31106030.39100.1636116.576
CD81 antigenCD81P600330.176115.2520.283315.252
β-1,3-N-acetylglucosaminyltransferase lunatic fringeLFNGQ8NES30.241557.1230.182314.752
Insulin-like growth factor-binding protein 2IGFBP2P180650.24740858.15170.17687859.3816
Kallikrein-6KLK6Q928760.2261540.9880.1653433.617
LactadherinMFGE8Q084310.2610618.6060.1137323.008
Integrin β-4ITGB4P161440.23422.6950.14541.102
UPF0556 protein C19orf10C19orf10Q969H80.2295330.0670.1382727.175
LumicanLUMP518840.20358.5830.125014.795
Integrin α-6ITGA6P232290.1757015.93150.15115517.1717
Laminin subunit γ-1LAMC1P110470.2294623.74330.09101411.2515
Ephrin-A3EFNA3P527970.12436.7210.191186.721
NeuroserpinSERPINI1Q995740.159720.9880.1314323.6611
Mesencephalic astrocyte-derived neurotrophic factorMANFP551450.1625643.02100.1246353.6312
Chitinase domain-containing protein 1CHID1Q9BWS90.186413.7450.097010.694
Interstitial collagenaseMMP1P039560.147915.3570.1320918.989
Kallikrein-10KLK10O432400.1047223.5550.1092130.077
NeogeninNEO1Q928590.0433916.50170.0223412.3215
  • a Ratios among proteins in the heavy and light versions were used as fold-change.