Table IV Competitive and transient binders of desthiobiotin-ATP in normal cultures
Gene no.Molecular massp valueAvg. NSAF_ATPγSaAvg. NSAF_ATPbFold change
Competitive Binders of Desthiobiotin-ATP
    Acetyl-/propionyl-CoA carboxylase α subunit, AccA1Rv2501c71 kDa(0.0000)1.0015.6715.67
    Glutamyl-tRNA amidotransferase subunit B, GatBRv3009c55 kDa(0.0000)2.3328.3312.14
    Inosine-5-monophosphate dehydrogenase, GuaB3Rv3410c39 kDa(0.0000)1.0011.6711.67
    Transmembrane serine/threonine-protein kinase H, PknHRv1266c67 kDa(0.0000)1.0011.3311.33
    Fatty-acid-CoA ligase, FadD7Rv011955 kDa(0.0000)1.009.679.67
    Phosphoribosylamine-glycine ligase, PurDRv077244 kDa(0.0000)1.009.339.33
    Tryptophanyl-tRNA synthetase, TrpSRv3336c36 kDa(0.0001)1.007.007.00
    Polyphosphate kinase, PpkRv298483 kDa(0.0000)3.3322.676.80
    Phosphoglycerate kinase, PgkRv143743 kDa(0.0000)3.0019.676.56
    Cysteinyl-tRNA synthetase 1, CysS1Rv3580c52 kDa(0.0005)1.005.675.67
    Fatty-acid-CoA ligase, FadD23Rv382663 kDa(0.0014)1.005.005.00
    Universal stress proteinRv2319c32 kDa(0.0012)1.336.334.75
    CTP synthase, PyrGRv169964 kDa(0.0024)1.004.674.67
    Phosphofructokinase, PfkBRv2029c35 kDa(0.0073)1.004.004.00
    ATP-dependent protease ATP-binding subunit, ClpC1Rv3596c94 kDa(0.0000)15.3361.003.98
    Anchored-membrane serine/threonine-protein kinase, PknFRv174651 kDa(0.0130)1.003.673.67
    Chaperone protein, HtpGRv2299c73 kDa(0.0130)1.003.673.67
    DNA polymerase I, PolARv162998 kDa(0.0078)1.676.003.60
    Acetate kinase, AckARv040941 kDa(0.0220)1.003.333.33
    UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diaminopimelate-d-alanyl-d-alanyl ligase, MurFRv2157c52 kDa(0.0220)1.003.333.33
    Conserved hypothetical proteinRv328223 kDa(0.0220)1.003.333.33
    Conserved hypothetical proteinRv2510c57 kDa(0.0220)1.003.333.33
    Fatty-acid-CoA ligase, FadD28Rv294163 kDa(0.0077)2.337.673.29
    Nucleoside diphosphate kinase, NdkARv2445c15 kDa(0.0150)2.006.333.17
    40-kDa secreted l-alanine dehydrogenase, AldRv278039 kDa(0.0066)3.009.333.11
    Adenosylhomocysteinase, SahHRv3248c54 kDa(0.0017)5.0015.003.00
    Pyruvate kinase, PykARv161751 kDa(0.0053)3.6711.003.00
    Malate synthase G, GlcBRv1837c80 kDa(0.0420)1.674.672.80
    Aldehyde dehydrogenaseRv045855 kDa(0.0350)2.677.002.63
    Adenylate kinase, AdkRv073320 kDa(0.0035)17.3336.672.12
    transmembrane serine/threonine-protein kinase D, PknDRv0931c70 kDa(0.0400)8.6717.672.04
    ATP synthase β chain, AtpDRv131053 kDa(0.0250)13.3326.331.98
Transient binders of desthiobiotin-ATP
    Endopeptidase ATP-binding protein chain B, ClpBRv0384c93 kDa(0.0410)25.3344.331.75
    3-Hydroxyacyl-thioester dehydrogenase, HtdYRv3389c30 kDa(0.4000)4.677.001.50
    Iron-regulated short-chain dehydrogenase/reductaseRv322430 kDa(0.4100)6.339.331.47
    Aminomethyltransferase, GcvTRv2211c40 kDa(0.4300)4.336.331.46
    Phosphopantetheine adenylyltransferase, KdtBRv2965c18 kDa(0.4700)2.333.331.43
    Chaperone protein, DnaKRv035067 kDa(0.3800)40.0057.001.43
    Cold shock protein A, CspARv3648c7 kDa(0.5300)5.677.671.35
    Secreted fibronectin-binding protein antigen85-B fbpBRv1886c35 kDa(0.5400)2.002.67
    Iron-regulated peptidyl-prolyl cis-trans isomerase, PpiARv000919 kDa(0.5400)3.004.001.33
    Glutamine synthetase, GlnA2Rv2222c50 kDa(0.5500)6.008.001.33
    Pyruvate dehydrogenase E2 component, SucBRv221557 kDa(0.5500)7.339.671.32
    Acyl-CoA dehydrogenase, FadE5Rv0244c66 kDa(0.5700)3.334.331.30
    Pyruvate dehydrogenase E1 component, AceERv2241100 kDa(0.5700)4.676.001.29
    60-kDa chaperonin 2, GroEL2Rv044057 kDa(0.0180)279.00352.001.26
    Iron-regulated conserved hypothetical proteinRv163615 kDa(0.3500)17.0021.001.24
    Propionyl-CoA carboxylase β chain 5, AccD5Rv328059 kDa(0.5800)3.003.671.22
    Low molecular weight protein antigen, Cfp2Rv2376c17 kDa(0.5000)4.675.671.21
    Glutamine synthetase, GlnA1Rv222054 kDa(0.3000)17.6721.331.21
    Heat shock protein, HspXRv2031c16 kDa(0.1800)36.0043.331.20
    Succinyl-CoA synthetase β chain, SucCRv095141 kDa(0.3500)11.6714.001.20
    Oligoribonuclease, OrnRv251123 kDa(0.4800)5.006.001.20
    Protein transport protei, SecE2Rv03798 kDa(0.5400)3.334.001.20
    l-Lactate dehydrogenase, LldD2Rv1872c45 kDa(0.4500)5.336.331.19
    Conserved hypothetical proteinRv2159c36 kDa(0.4300)5.676.671.18
    Bifunctional coenzyme A carboxylase α chain, AccA3Rv328564 kDa(0.2800)13.6716.001.17
    50 S ribosomal protein L7/L12, RplLRv065213 kDa(0.2500)16.0018.671.17
    Conserved hypothetical proteinRv326910 kDa(0.4800)4.004.671.17
    Ribosome recycling factor, FrrRv2882c21 kDa(0.5700)2.332.671.14
    Superoxide dismutase, SodARv384623 kDa(0.3900)5.336.001.13
    Transmembrane serine/threonine-protein kinase E, PknERv174361 kDa(0.3900)5.336.001.13
    Two-component system transcriptional regulator, DevRRv3133c23 kDa(0.4600)3.333.671.10
    Tetrahydrodipicolinate N-succinyltransferaseRv1201c33 kDa(0.4600)3.333.671.10
    Leucyl-tRNA synthetase, LeuSRv0041108 kDa(0.3800)4.334.671.08
    Iron-regulated elongation factor tu tufRv068544 kDa(0.0300)40.3343.331.07
    Immunogenic protein, Mpt64Rv1980c25 kDa(0.3000)6.006.331.06
    30 S ribosomal protein S1, RpsARv163053 kDa(0.2500)7.337.671.05
    Transmembrane serine/threonine-protein kinase A, PknARv0015c46 kDa(0.2100)8.679.001.04
    Transcriptional regulatorRv002327 kDa(0.5600)1.671.671.00
    Hypothetical protein, Wag31Rv2145c28 kDa(0.4200)2.672.671.00
    Hypothetical proteinRv381858 kDa(0.5000)2.002.001.00
    Conserved alanine-rich proteinRv2744c29 kDa(0.2800)5.005.001.00
    Enoyl-CoA hydratase, EchA9Rv1071c36 kDa(0.3900)3.003.001.00
    Conserved hypothetical proteinRv2140c19 kDa(0.5000)2.002.001.00
    Thiosulfate sulfurtransferase, CysA2Rv0815c31 kDa(0.5600)1.671.671.00
    Zinc-type alcohol dehydrogenase NAD-dependent, AdhBRv0761c40 kDa(0.2300)4.674.330.93
    Transcriptional regulator, MoxR1Rv147941 kDa(0.2800)3.333.000.90
    60-kDa chaperonin 1, GroEL1Rv3417c56 kDa(0.0270)16.3314.670.90
    Meromycolate extension acyl carrier protein, AcpMRv224413 kDa(0.1400)6.335.670.89
    Haloalkane dehalogenaseRv229633 kDa(0.1500)6.005.330.89
    Succinyl-CoA synthetase α chain, SucDRv095231 kDa(0.1600)5.675.000.88
    ATP synthase α chain, AtpARv130859 kDa(0.3300)2.332.000.86
    Integration host factor, MihFRv138821 kDa(0.0001)41.3334.670.84
    acyl-CoA dehydrogenase, FadE25Rv3274c42 kDa(0.3500)2.001.670.83
    DNA polymerase III β chain, DnaNRv000242 kDa(0.3500)2.001.67
    Pyridoxamine 5-phosphate oxidase, PdxHRv260725 kDa(0.1100)5.674.670.82
    10-kDa chaperonin, GroESRv3418c11 kDa(0.0000)50.3340.670.81
    Iron-regulated aconitate hydratase, AcnRv1475c102 kDa(0.1200)5.004.000.80
    30 S ribosomal protein S16, RpsPRv2909c17 kDa(0.3800)1.671.330.80
    Hypothetical protein, Cfp17Rv182717 kDa(0.2000)3.332.670.80
    Fructose-bisphosphate aldolase, FbaRv0363c37 kDa(0.2000)3.002.330.78
    Short-chain type dehydrogenase/reductaseRv014830 kDa(0.2000)3.002.330.78
    Transcriptional regulator, TetR familyRv014431 kDa(0.2000)3.002.330.78
    19-kDa lipoprotein antigen precursor, LpqHRv376315 kDa(0.0250)8.676.330.73
    Conserved hypothetical proteinRv2406c15 kDa(0.2100)2.331.670.71
    Electron transfer flavoprotein β subunit, FixARv3029c28 kDa(0.0039)13.679.670.71
    Enolase, EnoRv102345 kDa(0.1300)3.332.330.70
  • a Average spectral count of desthiobiotin-labeled peptides was in the presence of 500 μm ATPγS. A value of 1 indicates 0 spectral counts in the presence of excess ATP analog.

  • b Average spectral count of desthiobiotin-labeled peptides with no excess ATPγS.