Table I Antibodies and oligonucleotides used in PLA, for creating PLA probes and visualizing RCA products

Oligo is DNA oligonucleotide.

OligoDescriptionOligonucleotide vendorConjugated antibodyDNA sequence
ACircularization oligo longIntegrated DNA Technologies5′-phosphate-CTA TTA GCG TCC AGT GAA TGC GAG TCC GTC TAA GAG AGT AGT ACA GCA GCC GTC AAG AGT GTC TA
BCircularization oligo long (single version)Integrated DNA Technologies5′-phosphate-GTT CTG TCA TAC AGT GAA TGC GAG TCC GTC TAA GAG AGT AGT ACA GCA GCC GTC AAG AGT GTC TA
CCircularization oligo shortIntegrated DNA Technologies5′-phosphate-GTT CTG TCA TAT TTA AGC GTC TTA A
DTag-specific oligo for EGFRIntegrated DNA Technologies5′-phosphate-GTA TCT GCT TAT GTC GCC CG
ETag-specific oligo for HER2Integrated DNA Technologies5′-phosphate-AGC GAT CTG CGA GAC CGT AT
FTag-specific oligo for HER3Integrated DNA Technologies5′-phosphate-CTA GTG CTG GAT GAT CGT CC
GTag-specific detection oligoIntegrated DNA Technologies5′-Alexa 488-GTA TCT GCT TAT GTC GCC CG
HTag-specific detection oligoIntegrated DNA Technologies5′-Cy5-CTA GTG CTG GAT GAT CGT CC
ITag-specific detection oligoIntegrated DNA Technologies5′-Alexa 555-AGC GAT CTG CGA GAC CGT AT
JEGFR general PLA probe oligoTriLink BiotechnologiesEGFR Ab-15 (LabVision)5′-aldehyde-GAC GCT AAT AGT TAA GAC GCT T
KEGFR-specific PLA probe oligoTriLink BiotechnologiesEGFR Ab-15 (LabVision)5′-aldehyde-AAA AAA AAA ATA TGA CAG AAC CGG GCG ACA TAA GCA GAT ACT AGA CAC TCT T
LHER2 general PLA probe oligoTriLink BiotechnologiesHER2 Ab-8 (LabVision)5′-aldehyde-GAC GCT AAT AGT TAA GAC GCT T
MHER2-specific PLA probe oligoTriLink BiotechnologiesHER2 Ab-8 (LabVision)5′-aldehyde-AAA AAA AAA ATA TGA CAG AAC ATA CGG TCT CGC AGA TCG CTT AGA CAC TCT T
NHER3 general PLA probe oligoTriLink BiotechnologiesHER3 Ab-2 (LabVision)5′-aldehyde-GAC GCT AAT AGT TAA GAC GCT T
OHER3-specific PLA probe oligoTriLink BiotechnologiesHER3 Ab-2 (LabVision)5′-aldehyde-AAA AAA AAA ATA TGA CAG AAC GGA CGA TCA TCC AGC ACT AGT AGA CAC TCT T