Table III Competitive and transient binders of desthiobiotin-ATP in hypoxic cultures
Gene no.Molecular massp valueAvg. NSAF_ATPγSaAvg. NSAF_ATPb-Fold change
Competitive binders of desthiobiotin-ATP
    Fatty-acid oxidation protein, FadBRv086076 kDa(0.0039)16.336.33
    Inosine-5-monophosphate dehydrogenase, GuaB3Rv3410c39 kDa(0.0056)16.006.00
    Transcription termination factor ρRv129765 kDa(0.0079)15.675.67
    Macrolide-transport ATP-binding protein, ABC transporterRv2477c62 kDa(0.0160)15.005.00
    Endopeptidase ATP-binding protein chain B, ClpBRv0384c93 kDa(0.0031)2.511.674.67
    Iron-regulated heparin-binding hemagglutinin, HbhARv047522 kDa(0.0320)14.334.33
    Adenosylhomocysteinase, SahHRv3248c54 kDa(0.0065)5.518.333.33
    Chaperone protein, DnaKRv035067 kDa(0.0001)1650.003.13
    Adenylate kinase, AdkRv073320 kDa(0.0400)820.332.54
    Acyl-(acyl-carrier protein) desaturase, DesA1cRv0824c39 kDa(0.2200)24.672.33
    ATP synthase β chain, AtpDcRv131053 kDa(0.1500)511.332.27
    Bifunctional acetyl-/propionyl-coenzyme A carboxylase α chain, AccA3cRv328564 kDa(0.2600)36.332.11
Transient binders of desthiobiotn-ATP
    50 S ribosomal protein L7/L12, RplLRv065213 kDa(0.3100)4.58.671.93
    10-kDa chaperonin, GroESRv3418c11 kDa(0.3600)814.331.79
    ATP-dependent protease ATP-binding subunit, ClpC1Rv3596c94 kDa(0.5000)711.331.62
    60-kDa chaperonin 1, GroEL1Rv3417c56 kDa(0.5100)3.55.671.62
    Conserved hypothetical proteinRv262332 kDa(0.5600)3.55.331.52
    40-kDa secreted l-alanine dehydrogenase, AldRv278039 kDa(0.0520)3645.331.26
    Cold shock protein A, CspARv3648c7 kDa(0.4400)45.001.25
    60-kDa chaperonin 2, GroEL2Rv044057 kDa(0.0000)135168.671.25
    Heat shock protein, HspXRv2031c16 kDa(0.0002)67.575.001.11
    Transmembrane serine/threonine-protein kinase A, PknARv0015c46 kDa(0.3100)44.331.08
    Isocitrate lyase, IclRv046747 kDa(0.4300)2.52.671.07
    Integration host factor, MihFRv138821 kDa(0.0280)1920.001.05
    6-Phosphofructokinase, PfkARv3010c37 kDa(0.3200)3.53.671.05
    Bifunctional polyribonucleotide nucleotidyltransferase, GpsIRv2783c80 kDa(0.1600)6.56.671.03
    Iron-regulated elongation factor tu tufRv068544 kDa(0.0120)20.520.330.99
    Propionyl-CoA carboxylase β chain 5, AccD5Rv328059 kDa(0.5000)1.51.330.89
    Conserved hypothetical proteinRv326910 kDa(0.1600)4.54.000.89
    Electron transfer flavoprotein β subunit, FixARv3029c28 kDa(0.3300)21.670.83
    Conserved hypothetical proteinRv173811 kDa(0.0390)7.56.000.80
    Iron-regulated conserved hypothetical proteinRv163615 kDa(0.0093)107.330.73
    Transmembrane serine/threonine-protein kinase D, PknDRv0931c70 kDa(0.0100)63.330.56
    Conserved hypothetical proteinRv312739 kDa(0.0640)31.670.56
  • a Average spectral count of desthiobiotin-labeled peptides in the presence of 500 μm ATPγS. A value of 1 indicates 0 spectral counts in the presence of excess ATP analog.

  • b Average spectral count of desthiobiotin-labeled peptides with no excess ATPγS.

  • c p value of >0.05.