Table I Impacts of Salmonella SPI2 effectors on protein phosphorylation, a list of selected proteins

Asterisk represents ambiguous phosphorylation sites.

Gene nameProtein nameRAW264.7HeLa
Phospho siteRatio (ΔssaR/WT)Phospho siteRatio (ΔssaR/WT)
Protein trafficking (total 19)
    Arap1Arf-GAP with Rho-GAP domainSer-232*0.47
    RfflE3 ubiquitin-protein ligase RaftlinSer-2540.53
    EvlEna/VASP-like proteinSer-3270.55
    Msr1Macrophage scavenger receptor types I and IISer-22*0.56
    Sec22bVesicle-trafficking protein SEC22bSer-1370.66
    Snap23Synaptosome-associated protein 23Ser-110>10
Immune signaling (total 13)
    TyrobpTYRO protein-tyrosine kinase-binding proteinSer-900.40
    Marcksl1MARCKS-related proteinThr-140.44
    Mapk3ERK1Tyr-2050.54
    Ship1SHIP1Ser-9720.56
    Pak1CDC42/RAC effector kinase PAK1Thr-2250.59
    Mapk1ERK2Tyr-1850.61Ser-190*1.96
    rkcdPKCδSer-643*0.61
    PrkcaPKCα/β/γThr-4970.37
Cytoskeleton regulation (total 25)
    Cfl1Cofilin1Ser-30.47
    DstnDestrin (ADF)Ser-30.51
    Stmn1StathminSer-160.52
    Lsp1Lymphocyte-specific protein 1Thr-184*0.59
    Slc9a1Na+/H+ exchanger 1Ser-8010.62
    Hspb1HSP27Ser-150.19
    Cdc42ep1Cdc42 effector protein 1Ser-1210.40
    Lima1EplinSer-7260.52
    Sptbn1Spectrin β chain, brain 1Ser-14470.55
Others
    Herc4Probable E3 ubiquitin-protein ligase HERC4Thr-3290.21
    Znf828Zinc finger protein 828Ser-4160.21
    Rftn1RaftlinSer-243*0.29
    Fcho2FCH domain only protein 2Thr-494*0.30
    Sash1SAM and SH3 domain-containing protein 1Ser-830.32
    Eif3bEukaryotic translation initiation factor 3 subunit BSer-75*0.54Ser-1540.06
    Evi2bProtein EVI2BSer-269, Ser-2722.40
    Ppp1r2Protein phosphatase inhibitor 2Ser-1220.06
    Rune2Protein prune homolog 2Ser-595, Ser-5970.23
    Snd1EBNA2 coactivator p100Ser-4260.30
    Ttf1Transcription termination factor 1Ser-8722.55