Table II Selection analyses of basal snake and Iguania venom-encoding genes
SLACaFELbRELcIntegrative analysesMEME SitesdPAMLg
SLAC + FEL + REL + MEME
Basal SnakeM8M2a
ω>1e0265664020
Lectinsω<1f19095(16 + 24)(3 + 17)
ω =1.301.511.52
ω>1e0000
3FTxω<1f041011000
ω =0.480.770.77
ω>1e0001
ESPω<1f3309494100
ω =0.340.470.57
Iguania
ω>1e0000
Cystatinsω<1f0808000
ω =0.42480.550.47
  • 3FTx, Three-finger Toxin; ESP, Epididymal Secretory Protein.

  • a Single Likelihood Ancestor Counting.

  • b Fixed-effects likelihood.

  • c Random-effects likelihood.

  • d Sites detected as experiencing episodic diversifying selection (0.05 significance) by the Mixed Effects Model Evolution (MEME).

  • e Number of positively selected sites at 0.05 significance (for SLAC, FEL) or 50 Bayes factor (for REL).

  • f Number of negatively selected sites at 0.05 significance (for SLAC, FEL) or 50 Bayes factor (for REL).

  • g Positively selected sites detected using the Bayes Empirical Bayes approach implemented in M8 and M2a. Sites detected at 0.99 and 0.95 significance are indicated in the parenthesis; ω: mean dN/dS.