Table I Purification table of CS-0777-P phosphatase from the human kidney
StepProtein conc. [μg/ml]Activity [U/ml]Volume [ml]Total protein [mg]Total activity [U]Specific content [U/mg]Step fold changeOverall fold changeStep recovery [%]Overall recovery [%]
Human kidney homogenate1654357101655703.4-1-100
1) Centrifugation4339176104317604111.812309309
2) Multimodal anion exchange (Capto adhere)1881850.990962.4285.116
3) Anion Exchange (Mono Q)1014210.1424164.3121477.4
4) Gel filtration (Superdex 200)1070.050.00050.47111.72060.80.1