Table I Results of the protein–protein interaction studies using GFP-fusions of SIRK1, SIRK1S744A, and SIRK1S744D as bait under either sucrose-starvation conditions or sucrose-resupplied conditions for 3 min. Numbers indicate the protein abundance ratio of bait pull-down to GFP-only control and were obtained by averaging results from three biological replicates. Asterisks indicate significant differences (p < 0.05) after pairwise t tests of normalized ion intensity sums for bait pull-down versus control pull-down and after multiple-testing correction (64). The minimum p value across all conditions is shown; individual p values and standard deviations for the displayed averaged ratios are available in supplementary Table S4
InteractorNormalized ion intensity sums log2(bait/GFP-only)DescriptionMinimum
Sucrose starvationSucrose resupply (3 min)p value
SIRK1SIRK1S744DSIRK1S744ASIRK1SIRK1S744DSIRK1S744A(t test)
Aquaporins
    AT1G016200.6892.008*1.113*1.492*PIP1C, PIP1;31.56E-06
    AT2G371701.5531.359PIP2B, PIP2;20.052843
    AT2G390100.761*1.599*1.293*1.540*PIP2E, PIP2;65.29E-05
    AT3G534202.038*0.840*1.313*PIP2A, PIP2;11.29E-15
    AT3G614302.027*1.152*1.449*PIP1A, PIP1;19.26E-07
    AT4G004302.678PIP1E, PIP1;40.087501
    AT4G234000.7210.6921.965*1.298*1.126*PIP1D, PIP1;50.000336
    AT4G351001.979*1.119*1.434*PIP3A, PIP2;71.1E-13
    AT5G60660−0.7361.578*1.8172.338PIP2F, PIP2;40.040114
Kinases
    AT2G432302.075Protein kinase superfamily protein0.051732
    AT3G02880−0.7701.358*1.063Leucine-rich repeat receptor-like protein kinase0.040978
    AT3G148401.2491.406Leucine-rich repeat receptor-like protein kinase0.078647
    AT3G632602.506RAF-kinase ATMRK10.078331
    AT4G08850−1.5111.8302.291Leucine-rich repeat receptor-like protein kinase0.084065
    AT4G352301.479*1.5681.088BSK1 (BR-signaling kinase 1)0.025121
    AT5G465701.701BSK2 (BR-signaling kinase 2)0.064452
Remorins
    AT2G458201.078Remorin family protein0.076945
    AT3G612600.5441.537*1.165*1.074*Remorin family protein1.80E-06
Clathrins and coatomers
    AT2G207601.198*0.893*1.065*0.868*Clathrin light chain protein0.005237
    AT3G085301.039*−0.855*0.979*0.782*Clathrin, heavy chain0.000711
    AT3G111300.887*−0.688*0.946*0.506*0.631*Clathrin, heavy chain3.08E-43
    AT4G31480−0.745*1.217*0.700*0.750*Coatomer beta subunit2.85E-08
Other
    AT2G454700.935*0.5110.701*FLA80.000804
    AT1G038700.741*0.623*FLA90.007464
    AT2G20990−1.241*1.440*0.997*SYTA, synaptogamin A1.43E-06
    AT1G168902.117*1.500*1.784*UBC36, ubiquitin conjugating enzyme 362.47E-05
    AT1G721500.748*1.096*1.686*1.666*1.680*PATL1, Patellin12.78E-45
    AT1G225300.766*1.063*0.555*1.138*1.121*1.085*PATL2, Patellin21.18E-20
    AT1G418301.131*1.942*1.415*SKS60.016744
    AT4G202601.023*1.670*1.363*1.390*PCAP14.80E-24
    AT1G540000.515*0.523*0.946*0.684*GDSL-like lipase1.32E-09
    AT1G540100.586*0.774*1.176*0.994*GDSL-like lipase2.04E-16
    AT1G788301.264*0.723*1.378*1.895*2.167*Cucurlin-like lectin family protein2.49E-07
    AT1G788501.376*0.956*1.551*2.001*1.965*d-mannose binding lectin family protein3.21E-10
    AT4G35790−1.2801.376*0.9461.295*PLD delta, phospholipase D0.001986