Table II SLP76 interactome in activated BMMC. The proteins listed were recurrently identified in four affinity purifications of SLP76OST complexes in activated cells. Listed molecules were enriched at least fourfold in activated SlpOST/OST (OST+) compared to Slp+/+ BMMC (WT+). The enrichment was measured by the OST+/WT+ ratio and its significance (p < 10−3) determined using student's t-test. The enrichment existing between activated (OST+) and nonactivated (OST−) BMMC is also shown and its significance was determined using student's t-test (at least fourfold enrichment: **p < 10−2, ***p < 10−3). UniProt Accession numbers, protein symbols, and molecular function are also shown
Protein symbol Activated BMMCProtein nameUniProt AccFunctionRatio
OST+/WT+OST+/OST−
LAT2Linker for activation of T-cells family member 2Q9JHL0Adapter Protein71464820***
BCRBreakpoint cluster region proteinQ6PAJ1Kinase/GTPasa activation168148**
HIG1AHIG1 domain family member 1AQ9JLR9Unknown144104***
CDC23Cell division cycle protein 23 homologQ8BGZ4Cell division24555
DC1I2Cytoplasmic dynein 1 intermediate chain 2O88487Cytoskeleton regulation1538**
LAT1Linker for activation of T-cells family member 1O54957Adapter Protein3637**
PLCγ11-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1Q62077Phospholipase3636***
SHC1SHC-transforming protein 1P98083Adapter Protein2230***
DOK3Docking protein 3Q9QZK7Adapter Protein8,629***
SKAP2Src kinase-associated phosphoprotein 2Q3UND0Src Kinase regulator3422***
SUCASuccinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialQ9WUM5Ligase2022**
GRB2Growth factor receptor-bound protein 2Q60631Adapter Protein1816***
SKAP1Src kinase-associated phosphoprotein 1Q3UUV5Src Kinase regulator2214***
HECD3E3 ubiquitin-protein ligase HECTD3Q3U487Ligase4712
MSH2DNA mismatch repair protein Msh2P43247DNA-binding1211**
CSTF1Cleavage stimulation factor subunit 1Q99LC2mRNA processing1011**
FYBFYN-binding proteinO35601Adapter Protein179.5**
BTKTyrosine-protein kinase BTKP35991Kinase6,16.7**
PACS1Phosphofurin acidic cluster sorting protein 1Q8K212Coat protein135.4**
1433η14–3-3 protein etaP68510Adapter Protein264**
1433ϴ14–3-3 protein thetaP68254Adapter Protein133,8
Q99M59Interferon gamma inducible protein 47Q99M59Hydrolase activity6,93,7
CLNKCytokine-dependent hematopoietic cell linkerQ9QZE2Adapter Protein403,7
VAVProto-oncogene vavP27870Guanine-nucleotide releasing factor103,3
MA2C1Alpha-mannosidase 2C1Q91W89Mannosidase133,1
1433γ14–3-3 protein gammaP61982Adapter Protein453,0
1433ϵ14–3-3 protein epsilonP62259Adapter Protein202,8
E9Q4N7Protein Arid1bE9Q4N7Unknown282,4
1433β14–3-3 protein beta/alphaQ9CQV8Adapter Protein192,3
1433ζ14–3-3 protein zeta/deltaP63101Adapter Protein152,0
RHG27Rho GTPase-activating protein 27A2AB59GTPase activation1231,8
TP53BTumor suppressor p53-binding protein 1Q99LC2mRNA processing101,3
FA49BProtein FAM49BQ921M7Unknown291,2
GRAP2GRB2-related adaptor protein 2O89100Adapter Protein10860,9
RABE1Rab GTPase-binding effector protein 1O35551GTPase activation200,4