Table II Comparison of peptide and protein level KALIP for known SYK substrates identification. Abbreviations: BTK, bruton tyrosine kinase; PLCG2, 1- phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; GCET2, germinal center B-cell-expressed transcript 2 protein; HPK1, mitogen-activated protein kinase kinase kinase kinase 1; BLNK, B-cell linker protein; LAT2, linker for activation of T-cells family member 2; HS1, hematopoietic lineage cell-specific protein; TUBA, tubulin alpha
SubstratePhosphopeptidesPeptide KALIPProtein KALIPRatio(SYK+/SYK-)References
BTKRYVLDDEyTSSVGSK-Y2.5(58)
PLCG2MyVDPSEINPSMPQR-Y3(59)
GCET2GNSAEEYyENVPCKAYYQuantified other sites(60)
HPK1LSESSDDDyDDVDIPY--(55)
BLNKLLEDEADyVVPVEDNY--(57)
LAT2EDEESEDyQNSASIHWQRY--(56)
HS1EPEPENDyEDVEEMDRYYQuantified other sites(61)
TUBAMAALEKDYEEVGVDSY-Quantified other sites(62)