Table I Summary of the major mass spectrometry-based studies performed on mycobacterial secretome to date

Table includes details about the number of total proteins and secreted proteins, the analysis method, and the type of mass spectrometer used in the listed studies.

YearTitleNo. of identificationsNo. of secreted proteinsAnalysis method and instrument usedReference
1997Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry32n.d.2-DE and TSQ-700 quadrupole MS(25)
1999Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens263542-DE and MALDI-TOF (Voyager Elite MS)(26)
2000Toward the proteome of Mycobacterium tuberculosis288492-DE and MALDI-MS(27)
2000Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection61142-DE and microsequencing, immunodetection(28)
2001The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis30n.d.2-DE and LCQ-ESI-MS(29)
2002Hypoxic response of Mycobacterium tuberculosis studied by metabolic labeling and proteome analysis of cellular and extracellular proteins722-DE and LCQ-MS(30)
2003Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen13742MALDI-MS PMF, ESI-MS/MS or CapLC-ESI-MS/MS(8)
2003Comparative proteome analysis of culture supernatant proteins of Mycobacterium tuberculosis H37Rv and H37Ra5n.d.2-DE-MALDI-TOF MS(31)
2003Identification of novel proteins in culture filtrates of Mycobacterium bovis bacillus Calmette-Guérin in the isoelectric point range 6–1112n.d.2-DE and MALDI-TOF (Voyager Spec MS)(12)
2004CFP10 discriminates between nonacetylated and acetylated ESAT-6 of Mycobacterium tuberculosis by differential interaction841-DE and MALDI-MS and ESI-MS/MS (Voyager Elite)(10)
2007Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv2571592-DE and MALDI-TOF MS; LC coupled MS/MS.(11)
2009Immunoproteomic identification of secretory and subcellular protein antigens and functional evaluation of the secretome fraction of Mycobacterium immunogenum, a newly recognized species of the Mycobacterium chelonae-Mycobacterium abscessus group3342-DE and MALDI-TOF(33)
2009Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins41312-DE, Ligand Blotting and Immunoblotting, LC-3200 Q TRAP-MS(32)
2010The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins9n.d.2DE-3200 Q-TRAP-MS/MS(13)
2010Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis101n.d.2-DE,iTRAQ-labeled and LTQ-MS(34)
2011Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur101532-DE and MALDI-TOF/TOF (4700 Proteomics Analyzer)(14)
2012Characterization of the Mycobacterium tuberculosis proteome by liquid chromatography mass spectrometry-based proteomics techniques: a comprehensive resource for tuberculosis research398n.d.LC and ESI-MSMS (LTQ-Orbitrap Classic)(74)
2013Analysis of the secretome and identification of novel constituents from culture filtrate of bacillus Calmette-Guérin using high-resolution mass spectrometry2391851-DE and ESI-MSMS (LTQ-Orbitrap Velos)This study
  • ESI, electrospray ionization; PMF, peptide mass fingerprint; n.d., no data.