Table I Verification of candidate surface proteins. Nd: Not determined; IFA: Immunofluorescence analyses; Sap: Saponin; Pre: Prenylation Palm: Palmitoylation domain; SP: Signal peptide; TM: Transmembrane domain
Name abbr.Gene nameAccession numberDomains/putative localizationSurface proteomeWestern blotIFA–live trophozoitesIFA–fixed trophozoites
PBS-solublePBS non-soluble+ Sap.− Sap.
EhAct1Activator 1 40 kDa subunitXP_651156, XM_646064.1Palm+ndnd++
EhC2–1C2 domain containing proteinXP_655299, XM_650207.2None++++++
EhC2–3C2 domain proteinXP_654499, XM_649407.2None++++++
EhCoroninCoroninXP_654419, XM_649327.1Non-class. secreted+ndnd+ndnd
EhCP-A1Cysteine proteinaseXP_650156, XM_645064.2SP++++ndnd
EhCP-A2Cysteine proteinase 2XP_650642, XM_645550.2SP++++ndnd
EhCP-A5Cysteine proteinaseXP_650937, XM_645845.2SP++++ndnd
EhDnaJDnaJ family proteinXP_653489, XM_648397.1SP++
EhFeHydFe hydrogenaseXP_652839, XM_647747.2None++
EhFeSODIron-containing superoxide dismutaseXP_648827, XM_643735.2None++++++
EhGNBPGuanine nucleotide-binding protein subunit beta 2-like 1XP_657050, XM_651958None+++++-
EhGrainin1Grainin 1XP_650372, XM_645280.2SP+++++
EhHydAPutative iron hydrogenaseAAG31036, AF262400.1None+++ndnd
EhHypPHypothetical proteinXP_652420, XM_647328.1None+++ndnd
EhLectinGalactose-specific adhesin 170kD subunitXP_655415, XM_650323.2SP/TM+ndnd+++
EhP120Proliferating-cell nucleolar antigen p120XP_654993, XM_649901.1None++++++
EhPP2ASerine/threonine-protein phosphatase 2AXP_656214, XM_651122.2Non-class. secreted+ndnd+ndnd
EhPrxPeripheral membrane protein peroxiredoxinXP_647907, XM_642815.2Palm++++++
EhRab7DRab family GTPaseXP_651915, XM_646823.1Pre/Palm++++ndnd
EhRab7ERab family GTPaseXP_651202, XM_646110.2Pre/Palm+++++
EhRab7GRab family GTPaseXP_656477, XM_651385.1Pre/Palmndnd+++
EhRab7HRab family GTPaseXP_653414, XM_648322.1Pre/Palmndnd+ndnd
EhRab11ARab family GTPaseXP_653051, XM_647959.2Pre/Palm+ndnd+ndnd
EhRhoRho family GTPaseXP_654488, XM_649396.2None++++++
EhRubRubrerythrinXP_652131, XM_647039.2None++++
EhTRThioredoxin reductaseXP_655748, XM_650656.2None+ndnd+++
EhURE3-BPURE3-BP sequence specific DNA binding proteinAAG18423, AF291721.1Non-class. secreted++++