Table I qRT-PCR conditions and primer sequences for analysis of gene expression
GenePrimersAmplicon (bp)
ST3GAL15′-CAGAGATGGACGGTCACT-3′; 5′-CAACTGTGGTTTCTGACG-3′197
ST3GAL25′-GTGCCTCCGACTGGTTTG-3′; 5′-GAAGCGGTAGGGGTTCTC-3′191
ST3GAL35′-TATGCTTCAGCCTTGATG-3′; 5′-TTGGTGACTGACAAGATGG-3′164
ST3GAL45′-ATGTTGGCTCTGGTCCTG-3′; 5′-AGGAAGATGGGCTGATCC-3′176
ST3GAL55′-CAAAGCAAGATGAGAAGG-3′; 5′-AAACTTGGGACGACATTC-3′213
ST3GAL65′-ATGTCTATTGGGTGGCAC-3′; 5′-CGCACACAGAAAAGGGTG-3′189
ST6GAL15′-CTTGTTTTCCTGCTCAGA-3′; 5′-GCAAACAGAAGAAAGACCA-3′166
ST6GAL25′-ACGCTGCTGATTGACTCTTCT-3′; 5′-CACATACTGGCACTCATCTAA-3′160
ST6GALNAC15′-CTGGTCTTCTTTCTCTTCG-3′; 5′-GTTGAGGGCATTGTTCTCT-3′192
ST6GALNAC25′-CTTTGCCCTGTACTTCTCG-3′; 5′-CAGCACTGGAATGGAGAGA-3′205
ST6GALNAC35′-GGACAACCTGGTACAAAGT-3′; 5′-TATCTCATTTCCCACCTTC-3′174
ST6GALNAC45′-ACCTGCCTGGACCACCACT-3′; 5′-TCGGCACTGTCGATCTCAG-3′188
ST6GALNAC55′-TGGACGGATACCTCGGAGT-3′; 5′-GTCTGGTCAATCTGGGAGC-3′121
ST6GALNAC65′-ACCTACCCCTCAGCAGACG-3′; 5′-CTTGAGGTTGACAGGTCGG-3′179
ST8SIA15′-TACTCTCTCTTCCCACAGG-3′; 5′-GACAAAGGAGGGAGATTGC-3′149
ST8SIA25′-GTGGTCTTCCTCATCTTCG-3′; 5′-GAGGAGCCGTTTATTACAAC-3′140
ST8SIA35′-ATTCTCTCACCCAGGAACTC-3′; 5′-CAATCCGAACACTATTCTTG-3′141
ST8SIA45′-CAAGAACTGAGGAGCACC-3′; 5′-TTTCCAACCTTCTACATTGTG-3′140
ST8SIA55′-CCTTTGCCTTGGTGACCT-3′; 5′-CATGGACAGCACCTTCACT-3′152
ST8SIA65′-CGGCAAGCAGAAGAATATG-3′; 5′-GCTTTCCACCTCGTAACTC-3′126
GAPDH5′-CTCCTCCACCTTTGACGCTG-3′; 5′-TCCTCTTGTGCTCTTGCTGG-3′175