Table IV Differentially expressed GC cell envelope proteins identified by iTRAQ
AccessionNameGeneAverage ratio ± S.D.aSbTc
F62:FA1090MS11:FA10901291:FA1090
Outer membrane
    Q5F726Multidrug efflux pump channel proteinmtrE/NGO13631.08 ± 0.042.65 ± 0.020.93 ± 0.15YN
    Q5F6Q4Transferrin-binding protein AtbpA/NGO14952.93 ± 0.250.83 ± 0.090.67 ± 0.07YN
Periplasmic
    Q5F9E2Putative type IV pilin-like proteinNGO04560.14 ± 0.041.11 ± 0.110.98 ± 0.05NN
    Q5F724*Antibiotic resistance efflux pump component MtrCdmtrC/NGO13650.93 ± 02.98 ± 0.390.28 ± 0.06YY
Inner membrane
    Q5F725Antibiotic resistance efflux pump componentmtrD/NGO13641.08 ± 0.013.21 ± 0.510.69 ± 0.02NN
Unknown
    Q5F5N1LipoproteinNGO18892.56 ± 0.092.4 ± 0.453.35 ± 0.02NN
    Q5F865Putative peroxiredoxin family protein/glutaredoxinNGO09260.39 ± 0.10.35 ± 0.110.27 ± 0.07NN
    Q5F9E4Type IV pilus assembly protein PilWpilW/NGO04540.34 ± 0.090.24 ± 0.020.26 ± 0.06NN
Cytoplasmic
    Q5F5Q8Elongation factor Tutuf1/NGO18420.41 ± 0.10.24 ± 0.030.3 ± 0.03NN
    Q5F5T630S ribosomal protein S17rpsQ/NGO18300.64 ± 0.230.41 ± 0.040.45 ± 0.02NN
    Q5F5V350S ribosomal protein L17rplQ/NGO18173.65 ± 0.071.7 ± 0.161.4 ± 0.05NY
  • a Average ratio and standard deviation were calculated for differentially expressed proteins identified in both biological replica experiments with associated p < 0.05.

  • b Predictions of signal peptide cleavage site using SignalP server (47).

  • c Presence of twin-arginine signal peptide as determined by TatP (48).

  • d MtrC protein has been shown to be localized to the periplasm (111).