Table III Ubiquitous proteins identified by iTRAQ in the GC MVs
AccessionNameGeneAverage ratio ± S.D.aSbTc
F62:FA1090MS11:FA10901291:FA1090
Outer membrane
    Q5F5F6Adhesin mafA 1/4mafA1/NGO1067 mafA4/NGO19720.97 ± 0.241.34 ± 0.361.25 ± 0.26NN
    Q5F651LPS-assembly protein lptDlptD, imp, ostA/NGO17151.13 ± 0.251.5 ± 0.041.39 ± 0.27YN
    Q5F5V7Major outer membrane protein porin P.IBporB/NGO18121.35 ± 0.331.6 ± 0.261.1 ± 0.02YN
    Q5FAG7Pilus-associated proteinpilC/NGO00551.38 ± 0.130.55 ± 0.030.97 ± 0.05NY
    Q5F6Q7Phospholipase A1pldA/NGO14920.97 ± 0.121.34 ± 0.221.29 ± 0.17NN
    Q5F5W8Putative uncharacterized proteinbamA/NGO18010.91 ± 0.071.21 ± 0.061.21 ± 0.04YN
    Q5FAD2Type IV pilus biogenesis and competence protein pilQpilQ, omc/NGO00940.94 ± 0.11.34 ± 0.310.89 ± 0.15YN
Periplasmic
    Q5F601CatalasekatA/NGO17670.98 ± 0.450.93 ± 0.10.82 ± 0.25NN
Inner membrane
    Q5F823Thiol:disulfide interchange protein DsbDdsbD/NGO09781.24 ± 0.541 ± 0.341.44 ± 0.41NN
    Unknown
    Q5F5U930S ribosomal protein S13rpsM/NGO18210.97 ± 0.160.88 ± 0.130.91 ± 0.03NN
    Q5F6H4Adhesin mafA 2/3mafA2/NGO1393;mafA3/NGO15841.22 ± 0.20.84 ± 0.040.92 ± 0.02NN
    Q5F501LipoproteinNGO21390.6 ± 0.090.81 ± 0.271 ± 0.05YY
    Q5F9W2IgA-specific metalloendopeptidaseiga/NGO02751.28 ± 0.161.35 ± 0.241.14 ± 0.37YN
    Q5F932Putative carboxy-terminal processing proteaseNGO05720.8 ± 0.11.54 ± 0.051.32 ± 0.4YN
    Q5FA09Putative MafB-like proteinNGO02251.23 ± 0.370.94 ± 0.241.11 ± 0.05YN
    Q5FAC8Putative pilus assembly proteinpilM/NGO00980.92 ± 0.130.76 ± 0.150.68 ± 0.17NN
    Q5F576Putative uncharacterized proteinNGO20540.97 ± 0.220.69 ± 0.071.48 ± 0.18YN
  • a Average ratios and standard deviations were calculated for proteins ubiquitously expressed in both biological experiments.

  • b Predictions of signal peptide cleavage site using SignalP server (47).

  • c Presence of twin-arginine signal peptide as determined by TatP search engine (48).