Table I Human Cc novel confirmed protein targets validated through different orthogonal approaches
Protein nameProtein abbreviationSDS-PAGE and MALDI-TOF/TOF-MSBiFCMolecular dockingSPRNMRITC
Casein kinase II subunit βCSNKIIβ+-
Chaperonin containing TCP1 subunit 2CCT2+nd1
Coronin-like proteinCORO1A+-
Eukaryotic translation initiation factor 2 αaelF2α++++++
Fructose 1,6-bisphosphate aldolase AaALDOA+++nd4nd4nd4
Ribosomal protein S7RPS7+nd1
Tubulin β chainTUBB+-
Tumor rejection antigen 1Hsp90B1+nd2
14–3-3 epsilonaYWHAE++++++
Heterogeneus nuclear ribonucleoprotein C1/C2ahnRNP C1/C2++nd5+--
Histone-binding protein RBBP7RBBP7+nd3-nd3nd3nd3
Minichromosome maintenance complex 6MCM6++nd5nd4nd4nd4
Minichromosome maintenance complex 7MCM7+-
SET nuclear oncogeneaSET++++++
Acidic nuclear phosphoprotein 32BaANP32B+++-++
Heterogeneus nuclear ribonucleoprotein LhnRNP L+nd3
NucleolinNCL++nd5nd4nd4nd4
Nucleosome assembly protein 1-like 4NAP1L4+nd1
Ser/Thr kinase receptor associated proteinaSTRAP+++nd4nd4nd4
ATP synthase subunit βATP5β+nd1
Heat shock 70 kDa proteinaHSPA5++nd5+++
  • Notes: nd1, not determined because they are not apoptosis-related proteins; nd2, not determined because the interaction with Cc was previously discarded (41); nd3, not determined because their cDNAs were not available; nd4, not determined because they were not overexpressed as soluble recombinant proteins; nd5, not determined because their PDB coordinates were not available.

  • a Cc protein interactors validated in at least three out of six techniques.