Table III Correlation of age and glycan structures or traits measured by different methods in Vis cohort. Presented p values are corrected for multiple testing using Bonferroni correction. Significance level is set at p ≤ 5.3 × 10−4 (94 tests)
Glycan ClassGlycan Structure or TraitUPLC-FLR (Total IgG)MALDI-TOF-MSLC-ESI-MSxCGE-LIF (Total IgG)
IgG1IgG2&3IgG1IgG2&3
RpRpRpRpRpRp
Total IgG glycansFA20.5981.28E-460.5601.95E-390.6262.01E-520.5541.74E-380.6212.57E-510.5753.25E-42
FA2B0.5626.91E-400.4722.70E-260.4511.26E-230.5575.65E-390.5361.49E-350.5551.15E-38
FA2G1a−0.3474.57E-13−0.4995.44E-30−0.5854.47E-44−0.1171.00E+00−0.4051.56E-18−0.2551.83E-06
−0.1071.00E+00−0.0351.00E+00
FA2BG1a0.0261.00E+00−0.0851.00E+00−0.3961.49E-170.0531.00E+00−0.2268.25E-050.0471.00E+00
0.2964.63E-090.3301.32E-11
FA2G2−0.6465.71E-57−0.6091.06E-48−0.6384.02E-55−0.6172.15E-50−0.6342.92E-54−0.6261.77E-52
FA2BG2−0.3932.89E-17−0.3336.46E-12−0.5074.34E-31−0.4222.34E-20−0.5205.09E-33−0.3613.22E-14
FA2G1S10.0621.00E+00−0.0261.00E+00−0.4942.92E-290.1761.61E-02−0.3311.01E-110.0031.00E+00
FA2G2S1−0.5881.35E-44−0.5196.58E-33−0.5896.72E-45−0.5855.75E-44−0.6197.82E-51−0.5848.00E-44
Total IgG glycans-derived traitsFGS/(FG+FGS)−0.2591.08E-06−0.0811.00E+00−0.4465.31E-23−0.4367.63E-22−0.3722.79E-15−0.4572.17E-24
FGS/(F+FG+FGS)−0.5931.30E-45−0.3364.04E-12−0.5771.55E-42−0.5431.25E-36−0.5862.99E-44−0.5576.38E-39
FG1S1/(FG1+FG1S1)0.1942.84E-030.1131.00E+00−0.0721.00E+000.2522.85E-06−0.0741.00E+000.0151.00E+00
FG2S1/(FG2+FG2S1+FG2S2)0.2031.06E-030.0701.00E+00−0.1866.41E-03−0.0631.00E+00−0.1201.00E+000.0871.00E+00
Neutral IgG glycans-derived traitsG0n0.6261.68E-520.5854.42E-440.6393.36E-550.5862.70E-440.6384.71E-550.5981.38E-46
G1n−0.4732.05E-26−0.4952.13E-29−0.6055.94E-48−0.3732.33E-15−0.5804.40E-43−0.4061.31E-18
G2n−0.6385.51E-55−0.6056.26E-48−0.6378.22E-55−0.6181.15E-50−0.6482.48E-57−0.6221.24E-51
  • a These glycan structures are measured as two isomers with UPLC-FLR and xCGE-LIF (with galactose on 6- and 3-arm), but as single masses in the MS methods.