Table II Cell membrane integrity after beadfection with microspheres 5 (cell-cleavable linker) and microspheres 6 (cell-cleavable linker conjugated to GFP), as determined via propidium iodide staining
Time (h)SampleUnstained cells (%)
Control97.7 ± 2.7
48Microspheres 597.1 ± 2.5
Microspheres 698.3 ± 1.7
Control99.3 ± 0.7
72Microspheres 599.7 ± 0.2
Microspheres 699.8 ± 0.3
Control98.1 ± 0.9
96Microspheres 598.1 ± 1.4
Microspheres 698.9 ± 0.7
  • Cells were grown on coverslips, beadfected, incubated for time periods as indicated, and stained with propidium iodide as described in “Experimental Procedures.” Numbers represent the mean percentage ± S.D. of unstained cells calculated from three experiments performed independently in triplicate, using cells from three different passage numbers. Totals of 74 to 529 cells (48 h), 305 to 1140 cells (72 h), and 414 to 1193 cells (96 h) cells were counted for each field of view.