Table IV Quantitation of total OHPro present in lung protein extracts 1 and 3 weeks post-bleomycin. Total lung OHPro quantity from control animals (n = 6), early fibrotic animals (1 week post-bleomycin; n = 3), and late fibrotic animals (3 weeks post-bleomycin; n = 3). Values are means ± S.D. The percentage of total OHPro in each fraction was calculated for each experimental group (controls, bleomycin 1 week, bleomycin 3 weeks)
Experimental groupLung tissue fractionOHPro per lung (ng)OHPro per group (%)
ControlsNaCl soluble218 ± 470.05
ControlsSDS soluble636 ± 2520.15
ControlsGuanidine soluble17,242 ± 65694.14
ControlsInsoluble398,370 ± 179,90395.65
Bleomycin 1 weekNaCl soluble376 ± 1070.08
Bleomycin 1 weekSDS soluble1180 ± 2080.26
Bleomycin 1 weekGuanidine soluble18,299 ± 41404.03
Bleomycin 1 weekInsoluble434,746 ± 61,42995.63
Bleomycin 3 weeksNaCl soluble792 ± 2530.11
Bleomycin 3 weeksSDS soluble1178 ± 2330.17
Bleomycin 3 weeksGuanidine soluble18,055 ± 37272.60
Bleomycin 3 weeksInsoluble674,629 ± 185,99597.12