Table II Percentage of newly synthesized guanidine-soluble ECM proteins extracted from control and bleomycin-induced fibrotic lung tissue after 1 and 3 weeks of label. Values represent mean ± S.D. (n = 3) where protein data were available from three distinct biological samples
ProteinUniProt accession numberAverage F control, 1 week (%)Average F bleomycin, 1 week (%)Average F control, 3 weeks (%)Average F bleomycin, 3 weeks (%)
AgrinaA2ASQ111.1 ± 0.514.0 ± 2.730.7 ± 0.364.4 ± 6.9
BiglycanbP2865365.8 ± 4.286.7 ± 5.185.1 ± 2.295.9 ± 2.1
Collagen α-1(I) chainbP1108710.3 ± 1.721.4 ± 4.917.9 ± 2.452.7 ± 3.2
Collagen α-2(I) chainaQ0114910.3 ± 1.517.9 ± 3.217.6 ± 2.653.8 ± 2.3
Collagen α-1(V) chaincO882079.9 ± 2.425.3 ± 8.047.062.7
Collagen α-2(V) chaincQ3U9627.6 ± 0.127.2 ± 6.420.4 ± 4.358.7
Collagen α-1(VI) chainbQ048576.7 ± 1.018.3 ± 1.814.5 ± 0.158.9 ± 11.4
Collagen α-2(VI) chainaQ027889.319.1 ± 1.715.7 ± 1.662.1 ± 10.7
DecorinP2865489.6N/AN/A98.4
DermatopontinbQ9QZZ67.2 ± 1.022.3 ± 6.216.4 ± 1.464.3 ± 5.9
FibronectinbP1127677.4 ± 2.996.2 ± 3.076.4 ± 2.594.7 ± 2.3
Laminin subunit α-4aP9792720.7 ± 8.124.6 ± 4.844.9 ± 1.469.2 ± 4.2
Laminin subunit α-5bQ610018.6 ± 1.921.8 ± 1.023.5 ± 3.251.9 ± 4.0
Laminin subunit β-1bP0246913.9 ± 2.631.5 ± 6.945.6 ± 7.381.1 ± 8.7
Laminin subunit β-2aQ6129216.8 ± 3.725.5 ± 4.739.1 ± 1.170.9 ± 4.5
Laminin subunit γ-1aP0246814.0 ± 0.723.3 ± 2.042.2 ± 1.367.4 ± 3.8
Microfibril-associated glycoprotein 4aQ9D1H98.1 ± 0.611.7 ± 3.622.7 ± 3.161.0 ± 3.3
Nidogen-1aP1049316.3 ± 1.325.7 ± 5.044.2 ± 1.574.2 ± 4.8
Nidogen-2aO883229.4 ± 1.113.0 ± 4.728.6 ± 1.049.3 ± 10.1
PerlecanbQ0579322.5 ± 1.233.3 ± 7.351.5 ± 1.579.9 ± 1.9
ProlarginQ9JK5355.578.5 ± 6.876.6 ± 10.299.1
Protein–glutamine γ-glutamyltransferase 2bP2198144.7 ± 2.263.3 ± 6.386.2 ± 2.397.5 ± 2.4
  • a p < 0.05 at 3 weeks only.

  • b p < 0.05 at both time points.

  • c p < 0.05 at 1 week only.