Table I Glycopeptides from tryptic digest of natural grass pollen and dust mite allergens identified by bottom up mass spectrometry.
AllergenPeptideModificationsa
Phl p 1V27PPGPNITATYGDK40Hyp1; Pen1dHex1Hex2–3HexNAc2
I24PKVPPGPNITATYGDK40Hyp2; Pen1–4dHex1Hex 2–3HexNAc2
Phl p 5aA26DLGYGPATPAAPAAGYTPATPAAPAEAAPAGK58Hyp6–7; Pen0,2
Phl p 5.0103A26DLGYGPATPAAPAAGYTPATPAAPAGAEPAGK58Hyp0–7; Pen0–7
Phl p 5bA20DAGYAPATPAAAGAAAGK38Hyp2; Pen0–1
Der p 1N150QSLDLAEQELVDCASQHGCHGDTIPR176HexNAc1,2
E227ALAQTHSAIAVIIGIKDLDAFR249Hex
Der f 1N151TSLDLSEQELVDCASQHGCHGDTIPR177HexNAc1,2
R204PNSQHYGISNYCQIYPPDVKQIR227Hex
E228ALTQTHTAIAVIIGIKDLR247Hex
Der p 2G49KPFQLEAVFEANQNTKTAK68Hex1–4
C95PLVKGQQYDIK106Hex
G100QQYDIKYTWNVPK113Hex
I114APKSENVVVTVK126Hex
Der f 2G49KPFTLEALFDANQNTK65Hex1–9
C95PLVKGQQYDIK106Hex
G100QQYDIKYTWNVPK113Hex1,2
I114APKSENVVVTVK126Hex
  • a Variations in number of monosaccharide units shown in bold. Characters: - represent “between” and, represent “or”. Hyp, hydroxyproline; Pen, pentose; dHex, deoxyhexose; Hex, hexose; HexNAc, N-acetylhexosamine.