Table II Mca1p cleavage specificity. Occurrence of K/R residues in GluC and native peptidomic experiments according to the different strains used. The occurrence of D residue in tryptic digests (identified by semi-trypsin database search) is used as a control
ConditionsGluCNative peptidomicsTrypsin
K/R enrichment (%)K/R enrichment (%)D enrichment (%)
mca1Δ/Δ-F only0.8210.422.49
WT-F only8.3729.032.15
All conditions3.8420.181.70