Table II Quantification of synthetic heavy labeled histone peptides. The highlighted amino acids mean that they are heavy, for example, the heavy A has more of 4.0071 Da than the light A
Endogenous peptidesSynthetic peptidesRT(min)AreaRatio
TKQTAR(+1)(+2)(+1)(+2)
TKQTAR816.458408.733820.465410.73618.341.56E+090.156
TKme1QTAR830.474415.741834.481417.74421.586.72E+090.671
TKme2QTAR788.463394.735792.470396.73910.828.19E+080.082
TKme3QTAR802.478401.743806.485403.74710.827.15E+080.071
TphKQTAR896.424448.716900.431450.71918.222.07E+080.021
KSTGGKAPR(+1)(+2)(+3)(+1)(+2)(+3)
KSTGGKAPR1069.601535.304357.2051075.614538.311359.21023.22.98E+100.189
Kme1STGGKAPR1083.616542.312361.8771089.630545.319363.88225.912.72E+100.173
Kme2STGGKAPR1041.606521.307347.8741047.619524.314349.87816.611.14E+100.072
Kme3STGGKAPR1055.621528.314352.5451067.642534.325356.55316.465.69E+090.036
KacSTGGKAPR1055.584528.296352.5331061.598531.303354.53821.681.51E+100.096
KSTGGKacAPR1055.584528.296352.5331064.602532.805355.53921.931.66E+100.105
Kme1STGGKacAPR1069.601535.304357.2051075.614538.311359.21024.683.17E+100.201
Kme2STGGKacAPR1027.589514.299343.2021033.603517.306345.20614.955.73E+090.036
Kme3STGGKacAPR1041.606521.307347.8741051.625526.317351.21414.865.14E+090.033
KacSTGGKacAPR1041.568521.288347.8611047.582524.295349.86620.239.09E+090.058