Table I Summary of N-glycan masses and compositions for three Penicillium species. Calculated m/z values for predicted N-glycans as [M+H]+ and [M-H] are listed; for observed values refer to the figures. All compositions are as HxNyPE/PCz, other than for the four marked with an asterisk, are corroborated with MS/MS data as well as, in many cases, by chemical and enzymatic treatments as discussed in the text. Qualitative assessments of abundance, based on fluorescence intensities of HPLC fractions, are indicated by +++, ++, +, trace or n.d. (not detected); H is hexose, N, N-acetylhexosamine, PC phosphorylcholine and PE phosphoethanolamine
Composition[M+H]+[M-H]P. nordicumP. dierckxiiP. verrucosum
H4N21151.441149.43++nd
H5N21313.501311.48+++++
H5N2PE1436.511434.49nd+nd
H6N21475.551473.54+++++
H6N2PE1598.561596.55trace++nd
H7N21637.601635.59+++++
H6N2PC*1640.611638.59tracendnd
H7N2PE1760.611758.60trace++nd
H8N21799.661797.64+++++++
H7N2PC*1802.661800.65tracendnd
H7N2PE21883.621881.61nd+nd
H8N2PE1922.671920.65+++++nd
H7N2PEPC*1925.671923.65tracendnd
H9N21961.711959.70+++++++++
H8N2PE22045.672043.66++nd
H9N2PE2084.782082.76+++++++
H8N2PEPC*2087.722085.71tracendnd
H10N22123.762121.75++++++
H9N2PE22207.732205.71trace+nd
H10N2PE2246.772244.76+++++++
H11N22285.822283.80++trace++
H10N2PE22369.782367.77nd+nd
H11N2PE2408.822406.81+++++
H12N22447.872445.85+trace++
H10N2PE32492.792490.77nd+nd
H11N2PE22531.832529.82+tracend
H12N2PE2570.882568.86tracetrace++
H13N22609.922607.91+trace+
H13N2PE2732.932730.92tracetrace+
H14N22771.972769.96tracend+
H14N2PE2894.982892.97tracendnd
H15N22934.032932.01tracendtrace
H16N23096.083094.06tracendtrace