Table I Summary of differentially expressed proteins in GA-treated HeLa cells compared with control for 3 hour treatment (from Spot 1 to Spot 9) or 24 hour treatment (from Spot 10 to Spot 18). *p < 0.05 vs the control group. The fold difference was represented by the ratio of the intensity value of GA-treated group to the value of the control group
Spot numberProtein nameSpot volume 3 h (ppm) (Mean ± S.E.)Fold difference (3 h)Spot volume 24 h (ppm) (Mean ± S.E.)Fold difference (24 h)Pairs of gels (n)
Control 3 hGA-treated 3 hControl 24 hGA-treated 24 h
1Heat shock 27kDa protein 11398.48 ± 183.52825.11 ± 167.50*0.59595.39 ± 121.67470.27 ± 177.510.799
2Calumenin79.14 ± 9.80008.57 ± 3.55*000.11159.34 ± 19.3091.61 ± 19.250.579
3Vimentin1427.08 ± 232.59763.68 ± 201.54*0.541534.01 ± 253.54948.69 ± 106.890.629
4Eukaryotic translation elongation factor 1 beta 23696.80 ± 489.882017.80 ± 355.32*0.553437.39 ± 594.303143.14 ± 816.550.919
5DJ-1 protein5835.58 ± 908.473125.98 ± 579.06*0.543061.27 ± 506.972509.36 ± 356.850.829
6BAG 2378.24 ± 102.91140.18 ± 38.37*00.37334.34 ± 58.37164.57 ± 53.190.499
7Chaperonin containing TCP1, subunit 3 (gamma)330.93 ± 43.130169.54 ± 30.70*00.51470.71 ± 55.28295.93 ± 52.770.639
8Nucleosome assembly protein 1-like 4479.41 ± 70.010279.63 ± 58.41*00.58404.47 ± 93.57432.61 ± 108.691.079
9Transaldolase 1139.42 ± 30.680331.68 ± 66.95*02.38245.68 ± 25.44381.61 ± 51.061.559
10Ribosomal protein, large, P0372.08 ± 117.75489.48 ± 156.171.32375.91 ± 94.040901.86 ± 200.18*2.409
11Vimentin1120.24 ± 154.022101.91 ± 436.001.881020.91 ± 535.062658.72 ± 412.91*2.609
12Nascent-polypeptide-associated complex alpha polypeptide1647.08 ± 209.191199.29 ± 294.100.731641.53 ± 330.63685.21 ± 144.82*0.429
13Gelsolin-like capping protein594.03 ± 100.26519.42 ± 61.650.87929.04 ± 151.96418.91 ± 106.75*0.459
14Keratin 18375.44 ± 47.48367.77 ± 49.970.98518.90 ± 112.78236.01 ± 63.60*00.459
15Eukaryotic translation elongation factor 1 delta isoform 2659.33 ± 110.97860.26 ± 98.141.30368.70 ± 78.100803.61 ± 112.32*2.189
16Beta actin292.80 ± 27.70269.6 ± 81.650.92267.26 ± 62.810101.89 ± 21.65*00.389
17Tubulin alpha 6236.27 ± 20.92337.42 ± 85.021.43243.86 ± 65.040990.48 ± 175.03*4.069
18Keratin 8759.39 ± 112.721032.57 ± 140.881.36190.11 ± 151.6001184.11 ± 299.06*6.239