Table III Transgenic animal models of AD where proteomic analysis has been performed
Model/genetic backgroundAges analyzedSampleType of analysis and reference
TgCRND8. Human APP 695 gene with both, Swedish/Indian mutation found in FAD under the prion promotor. C57BL/6 X C3H hybrid back-ground.2 and 6 monthsHippocampusPhosphopeptides enrichment with triple isotopic dimethylation labeling combined with online multidimensional separation and MS (145)
Double knock-in mice (APP/PS1). APP Swedish mutant with humanized Aβ sequence a proline to leucine mutation in residue 264 (P264L) was introduced in PS1. This mutation is identified in human FAD. CD-1/129 background.1, 6, 9, 12, and 15 monthsBrain2D-E/immunodetection of carbonylated proteins (100)
Tg2576. Human APP 695 with Swedish mutation (double mutations K670N and M671L). 129S6 background.6–8 monthsSynaptosomal mitochondriaDifferential 2D electrophoresis (DIGE)-MS (97)
Tg2576.Brain2-DE followed by MALDI-TOF and/or LC-MS/MS (146)
Thy1-APP751. These mice bear a transgenic construct containing the human APP 751 with the London and Swedish mutations under control of the murine Thy1 promoter. C57Bl6 background.14 monthsCortex pooledDifferential 2D electrophoresis (DIGE)-MS (147)
3 × TgAD. A triple-transgenic mouse model of FAD expressing mutant PS1 (M146V), APP Swedish mutant and mutant tau (P301L). 129X1/SvJ x 129S1/Sv-F1 background.14 monthsBrain/Cerebellum2D-E and MS (98)
3 × TgAD16 monthsHippocampus /CortexiTRAQ stable- isotope-based quantitative proteomic (148)
APP/PSEN1 double mutant mouse. Express APP Swedish and PSEN1 with the exon-9 deletion under the control of the mouse prion-protein promoter. C57/6J background.5 monthsBrainTandem mass tag-based quantitative proteomic analysis (149)
3 × TgAD6 monthCortex Mitochondria2D-DIGE and tandem MS (99)
APPE693delta. Human APP695 with the APPE693delta mutation under the mouse prion promoter.12 monthsHippocampus2D-DIGE and LC–MS/MS (125)
Double knock-in mice (APP/PSEN1)9–12 monthsBrain2D PAGE, in-gel trypsin digestion, and MS (102)
J20 Tg. mouse model of FAD carrying the Swedish and Indiana familial forms of FAD.Brain2D PAGE, in-gel trypsin digestion, and MS (103)
J20Tg and J20T M631L Tg AD mice.Brain2D PAGE, in-gel trypsin digestion, and MS (105)