Table I Candidate ceramide-binding protein fragments that meet FPKM values and enrichment cutoffs in both the bead-enriched and FACS-enriched sequencing data

FPKM values for the starting library, bead-enriched, and FACS-enriched are shown, and candidates are ranked by highest FPKM value of the enriched populations. The maximum enrichment was calculated by dividing the highest FPKM-enriched population value by the starting library value. Displayed amino acids predicted from the sequencing data, and the total amino acid counts of the full-length protein are shown. The names and accession numbers (in parentheses) of domains identified by searches of the NCBI conserved domain database, SMART domain database, and Pfam domain database are shown (RPN13-C, UCH-binding domain; EFh, EF-hand; SCP2, SCP-2 sterol transfer family; CX9C, CHCH-CHCH-like; SapB-1, saposin-like type B, region 1; SapB-2, saposin-like type B, region 2; STI1, heat shock chaperonin-binding motif; PAH, paired amphipathic helix repeat; PAM2, ataxin-2 C-terminal region; TPR, tetratricopeptide repeat; CRIB, Cdc42/Rac interactive binding; TAFH, NHR1 homology to TAF; PH-like, pleckstrin homology-like domain; UBQ, ubiquitin-like; Rod-C, rough deal protein C-terminal region).

GeneLibraryBead-enrichedFACS-enrichedMaximum enrichmentAmino acidsTotal amino acidsDomain
ADRM11508100,90728,83467262–407407RPN13-C (PF16550.1)
HPCA75954,15834,201711–1931933× EFh (cl08302)
HSD17B473574717,975245445–596596SCP2 (cl01225)
CHCHD57310,4876331442–110110CX9C (PF16860.1)
NCS18568721977812–1901903× EFh
RPLP258766903673111–115115Ribosomal-P2 (cl21508)
PSAP236623283726164–318524SapB-1 (cl04972), SapB-2 (cl08395)
RAD23A177609155334202–363363XPC-binding (cl18177), UBA-like (cl21463), STI1 (SM00727)
CABP158554340695237–3703703× EFh
PDCD6433798108881–1911915× EFh
MYL6B48324031668121–2082083× EFh
SIN3A34323010195109–2441273PAH (cl07842)
PAIP116266119016892–233479PAM2 (cl06258), MIF4G (cl02652)
SGTA67222726163988–3133133× TPR (cl22897)
CHP1522474148481–91195EFh
HBZ0.031123781–142142Globin-like (cl21461)
NCALD27199674741–851932× EFh
PDE6D389521871491–88150GMP-PDE-δ (cl12307)
RPS29381790113471–5656
WASL129068174217–337505CRIB (cl00113)
IKBIP9.581379861–87377
PDIA62614967827192–326492Thioredoxin-like (cl00388)
ST139.452546256301–369369STI1
RBP1234932582118–197197Lipocalin (cl21528)
HNRNPUL15843419381–64285
GDAP1L12.0251551271–179367Thioredoxin-like
MDM11.1200321761–92714MDM1 (cl21312)
TAF40.818027227590–7171085TAFH (cl02658)
GIGYF11113116014479–701754
ST13P41.7136467997–3693693× TPR, STI1
DAB21.3221219135–221235PH-like (cl17171)
UBQLN16.91001101695–226449UBQ (cl00155), STI1
UBQLN26.971102151–310624UBQ, 2× STI1
SCP21.9776541132–289289SCP2
KNTC10.217362082094–22092209Rod-C (cl20400)
BTG10.33.43011416–140171BTG (cl15439)