Table II Extracted oligosaccharides from infant feces. Neutral mass, composition given as Hex_HexNAc_Fuc_Neu5Ac, oligosaccharide type, volume, and retention time are provided based on nanoLC-MS and nanoLC-MS/MS analysis
Neutral massCompositionOligosaccharide typeVolumeRetention time (min)
750.2912_2_0_0HMO digest2796109.5
3377.2406_6_4_2Fucosylated and sialylated complex1962269.8
1829.6904_5_1_0Fucosylated complex/hybrid3790410.0
1464.5603_4_1_0Fucosylated complex13825110.1
1261.4803_3_1_0N-glycan digest54948710.8
1407.5403_3_2_0Fucosylated complex13725910.8
1407.5403_3_2_0Fucosylated complex15305511.5
1204.4603_2_2_0HMO digest50255811.7
896.34902_2_1_0HMO digest18208511.8
1407.5403_3_2_0Fucosylated complex4954411.8
1439.5305_3_0_0Intact HMO18415212.0
1058.4003_2_1_0HMO digest57453812.1
1204.4603_2_2_0HMO digest210331212.1
1220.4504_2_1_0Intact HMO238138212.1
1772.6704_4_2_0Fucosylated complex/Hybrid5736212.1
2354.82011_2_1_0Fucosylated high mannose21223912.2
1569.5904_3_2_0Fucosylated complex/hybrid20041712.3
1843.63010_1_0_0N-glycan digest10680612.4
1521.5803_5_0_0Complex10067212.5
1115.4203_3_0_0HMO digest or complex/hybrid98591412.6
1261.4803_3_1_0N-glycan digest23181612.6
912.3433_2_0_0HMO digest189175112.9
1058.4003_2_1_0HMO digest454912212.9
1204.4603_2_2_0HMO digest574995212.9
896.34902_2_1_0HMO digest12344013.0
2178.8304_6_2_0Fucosylated complex59533413.0
3579.3006_7_2_3Fucosylated and sialylated complex105956813.0
1667.6403_5_1_0Fucosylated complex9454013.2
1382.5105_2_1_0Intact HMO or fucosylated complex/hybrid4787913.3
896.3492_2_1_0HMO digest59687013.4
1261.4803_3_1_0N-glycan digest24084313.4
1407.5403_3_2_0Fucosylated complex17528513.4
547.2112_1_0_0HMO digest88132413.6
1058.4003_2_1_0HMO digest382427013.6
3465.2606_5_4_3Fucosylated and sialylated complex12422513.6
1220.4504_2_1_0Intact HMO1718548613.7
1115.4203_3_0_0HMO digest or complex/hybrid94718414.3
1261.4803_3_1_0N-glycan digest905756114.3
1058.4003_2_1_0HMO digest329260814.4
1204.4603_2_2_0HMO digest128507914.4
1569.5904_3_2_0Fucosylated complex/hybrid31499714.5
2428.9005_5_2_1Fucosylated and sialylated complex/hybrid27309714.5
547.2112_1_0_0HMO digest88407814.6
912.3433_2_0_0HMO digest7188414.6
1382.5105_2_1_0Intact HMO or fucosylated complex/hybrid382070214.6
1991.7405_5_1_0Fucosylated complex/hybrid8366915.0
2210.8206_6_0_0Complex20948615.0
2194.8205_6_1_0Fucosylated complex70823615.2
1204.4603_2_2_0HMO digest45599215.9
1893.7006_3_2_0Fucosylated hybrid11476715.9
1204.4603_2_2_0HMO digest61550516.1
1934.7205_4_2_0Fucosylated complex/hybrid15727316.1
1569.5904_3_2_0Fucosylated complex/hybrid647905216.4
1731.6405_3_2_0Intact HMO25030616.4
2908.1004_6_5_1Fucosylated and sialylated complex61479416.4
1626.6104_4_1_0Fucosylated complex/hybrid98964516.5
1261.4803_3_1_0N-glycan digest75846816.6
912.3433_2_0_0HMO digest10860016.7
1115.4203_3_0_0HMO digest or complex/hybrid147132616.7
1480.5504_4_0_0Complex/Hybrid96368916.7
1843.63010_1_0_0N-glycan digest32101216.7
2073.8003_7_1_0Fucosylated complex207795216.7
1261.4803_3_1_0N-glycan digest53554816.8
1756.6703_4_3_0Fucosylated complex10976916.8
1423.5304_3_1_0Intact HMO972819416.9
1569.5904_3_2_0Fucosylated complex/hybrid77914916.9
1731.6405_3_2_0Intact HMO62801816.9
1893.7006_3_2_0Fucosylated hybrid15292917.0
693.2692_1_1_0HMO Digest17077417.2
4267.5427_6_5_4Fucosylated and sialylated complex70827717.2
4121.4807_6_4_4Fucosylated and sialylated complex49118217.3
1724.6603_6_0_0Complex17558317.6
1714.6304_3_1_1Fucosylated and sialylated complex70562317.7
2908.1004_6_5_1Fucosylated and sialylated complex34461717.8
2997.1106_7_2_1Fucosylated and sialylated complex10619517.8
1115.4203_3_0_0HMO digest or complex/hybrid128081417.9
1626.6104_4_1_0Fucosylated complex/hybrid13794118.0
1731.6405_3_2_0Intact HMO225049918.0
3306.2407_7_5_0Fucosylated complex411215518.0
1519.5308_1_0_0N-glycan digest13303318.1
2046.72010_2_0_0High mannose10483118.4
1439.5305_3_0_0Intact HMO120659018.5
1585.5905_3_1_0Intact HMO965234918.5
1683.6304_5_0_0Complex/Hybrid90057718.5
1074.4004_2_0_0Intact HMO73650618.6
547.2112_1_0_0HMO digest29527118.8
1058.4003_2_1_0HMO digest28507118.8
2354.82011_2_1_0Fucosylated high mannose73372218.8
1731.6405_3_2_0Intact HMO649977419.0
1626.6104_4_1_0Fucosylated complex/hybrid31716719.2
2063.7704_4_2_1Fucosylated and sialylated complex8242819.2
2080.7805_4_3_0Fucosylated complex/hybrid22190019.4
2242.8306_4_3_0Intact HMO199564619.4
1033.3705_1_0_0N-glycan digest6570519.6
2354.82011_2_1_0Fucosylated high mannose25374519.8
1074.4004_2_0_0Intact HMO13340820.0
1115.4203_3_0_0HMO Digest or complex/hybrid86303020.0
1569.5904_3_2_0Fucosylated complex/hybrid52284420.1
2242.8306_4_3_0Intact HMO59626720.1
2137.8005_5_2_0Fucosylated complex/hybrid35430720.3
1407.5403_3_2_0Fucosylated complex35227920.4
1439.5305_3_0_0Intact HMO472769420.5
1585.5905_3_1_0Intact HMO141782920.5
2354.82011_2_1_0Fucosylated high mannose18608120.7
2980.0905_7_1_2Fucosylated and sialylated complex5808920.7
750.2912_2_0_0HMO digest51479620.8
1115.4203_3_0_0HMO digest or complex/hybrid34846820.8
1261.4803_3_1_0N-glycan digest313183420.8
1318.5003_4_0_0Complex27255320.8
1480.5504_4_0_0Complex/hybrid43032020.8
1382.5105_2_1_0Intact HMO35141920.9
1934.7205_4_2_0Fucosylated complex/hybrid35633120.9
1714.6304_3_1_1Fucosylated and sialylated complex75769121.0
1788.6705_4_1_0HMO digest or fucosylated complex/hybrid8021921.0
2957.1007_6_4_0Fucosylated complex15253021.1
1480.5504_4_0_0Complex/Hybrid67367021.2
1917.7104_4_1_1Fucosylated and sialylated complex6722521.2
1439.5305_3_0_0Intact HMO37353421.4
1480.5504_4_0_0Complex/Hybrid107486821.8
1568.5704_3_0_1Sulfated complex12455121.8
1788.6705_4_1_0HMO digest or fucosylated complex/hybrid15453121.8
1934.7205_4_2_0Fucosylated complex/hybrid13269422.3
2096.7806_4_2_0Intact HMO or fucosylated hybrid433623222.4
2137.8005_5_2_0Fucosylated complex/hybrid48963222.6
2615.9804_6_3_1Fucosylated and sialylated complex4930722.9
1480.5504_4_0_0Complex/Hybrid18983923.1
1845.6905_5_0_0Complex/Hybrid42955723.1
1950.7206_4_1_0Intact HMO355124823.2
2022.7405_3_2_1Intact HMO32944623.3
1439.5305_3_0_0Intact HMO15253623.4
1714.6304_3_1_1Fucosylated and sialylated complex38278123.4
2210.8206_6_0_0Complex19416423.4
2323.8704_6_1_1Fucosylated and sialylated complex4142623.5
1585.5905_3_1_0Intact HMO94505423.6
1480.5504_4_0_0Complex/Hybrid127531223.8
1804.6606_4_0_0Intact HMO18845523.8
3272.2105_7_3_2Fucosylated and sialylated complex9195123.8
2251.8405_7_0_0Complex8362824.1
1569.5904_3_2_0Fucosylated complex/hybrid40315124.2
2022.7405_3_2_1Intact HMO5190124.2
1845.6905_5_0_0Complex/Hybrid35243324.3
1876.6805_3_1_1Intact HMO11557824.3
2251.8405_7_0_0Complex19957024.3
1569.5904_3_2_0Fucosylated complex/hybrid8116624.6
1318.5003_4_0_0Complex6254325.2
1934.7205_4_2_0Fucosylated complex/hybrid9570526.1
1876.6805_3_1_1Intact HMO16819826.5
1730.6205_3_0_1Intact HMO or sialylated hybrid49731826.8
2095.7606_4_0_1Intact HMO or sialylated hybrid48188427.1
2219.8603_7_2_0Fucosylated complex38062027.8
1204.4603_2_2_0HMO digest4676428.0