Table I 2-Oxohistidine interacting proteins identified by E. coli K12 proteome chips. There were 38 and 20 proteins are identified by Oxo-AG peptide and Oxo-SE peptide chip assays, respectively. There were 10 proteins which showed affinity toward both peptide probes, as shown below
Accession IDProtein SymbolProtein NameProtein Function
EG11001thrSThreonyl-tRNA synthetaseAn enzyme involved in protein synthesis, which is regulated by aerobic and anaerobic metabolisms
EG12746yqjGGlutathionyl-hydroquinone reductaseReduction of organic small molecules
EG13272yajLAnti-oxidative stress chaperoneA covalent chaperone for thiol-containing proteome, also promoting disulfide formation
EG11543hemEUroporphyrinogen decarboxylaseInvolved in the synthesis of heme group, which is a critical cofactor for antioxidant enzymes
EG10493ilvAThreonine dehydrataseA metabolic enzyme that converts threonine to 2-oxobutanoate, regulated by an oxygen-responsive promoter
EG13603prpD2-Methylcitrate dehydrataseA metabolic enzyme in the methylcitrate cycle that converts propionyl-CoA to pyruvate
EG11221zwfGlucose-6-phosphate dehydrogenaseA metabolic enzyme in the pentose-phosphate pathway that supplies reducing power to cells by generating NADPH
EG10256edaKDPG aldolaseAn enzyme in the Entner-Doudoroff pathway, also a multi-function aldolase to detoxify aldehydes generated by oxidative stress
EG10412gorGlutathione reductaseAn enzyme that generates glutathione to maintain a reducing environment in the cell
EG11744pqqLPutative periplasmic M16 family zinc metalloendopeptidaseAn enzyme involved in pyrroloquinoline quinone biosynthesis, which is a redox cofactor that supplies reducing power