Table I List of putative T. urticae salivary proteins identified in artificial diet using nano-LC- MS/MS. Proteins are ranked based on normalized abundance factor (rTop3). See supplemental Table S6 for a detailed overview
T. urticae protein IDaFunctional descriptionMax. rTop3bSPcWPdISHeDffOrthoMCL (#)gFeeding FChProterosoma FCi
tetur01g01850Projectin0.1484NC*OG5_126738 (10)0.42.9*
tetur20g00560Hypothetical protein0.1137YEATu_MCL_12 (35)6.033.9*
tetur07g00150Serine protease0.0877YEA*OG5_135950 (7)8.028.6*
tetur28g01330, tetur55g00040Wannes-Thomas Secreted Protein (WTSP) 10.0855YETu_MCL_36 (13)3.737.1*
tetur03g03700Hypothetical protein/PE-PGRS family protein0.0817YEOG5_144177 (6)30.430.9*
tetur12g03940, tetur12g03950Serine protease0.0816YE*OG5_168371 (5)emb(0)10.4*
tetur03g03680Hypothetical protein/PE-PGRS family protein0.0738YEOG5_144177 (6)17.938.9*
tetur32g00050Small Secreted Protein, Family F0.0691YEATu_MCL_35 (14)2.310*
tetur03g03670, tetur03g03690, tetur03g03730, tetur03g10093Hypothetical protein/PE-PGRS family protein0.0628YEOG5_144177 (6)15.340*
tetur06g06630Cystatin0.0530YEATu_MCL_45 (11)emb(0)(−)
tetur06g02580Cathepsin B0.0469YE*OG5_127800 (28)1.55.9
tetur05g04580Wannes-Thomas Secreted Protein (WTSP) 100.0429YEDTu_MCL_36 (13)11.49.5*
tetur16g03470Serine protease homologue0.0387YE*OG5_152237 (2)3.712.6*
tetur06g03320Wannes-Thomas Secreted Protein (WTSP) 30.0328YETu_MCL_36 (13)(−)−3.3
tetur16g03420Beta-mannosidase0.0313YE*OG5_129300 (4)2.518.4*
tetur29g01360*Hypothetical protein0.0233YED3.829.5*
tetur03g10083Hypothetical protein/PE-PGRS family protein0.0219YETu_MCL_74 (6)9.436.1*
tetur01g02670MD-2-related lipid-recognition domain protein0.0217YE*OG5_158831 (2)4.6−2*
tetur04g05980*Apolipoprotein D precursor0.0215YE*OG5_130527 (50)6.4−1.3
tetur05g04560Wannes-Thomas Secreted Protein (WTSP) 80.0209YETu_MCL_36 (13)10.310.5*
tetur07g00160*Hypothetical protein0.0200YED4.414.6*
tetur03g03680, tetur03g03700, tetur03g10083Hypothetical protein/PE-PGRS family protein0.0196YEOG5_144177, Tu_MCL_74 (6)17.938.9*
tetur40g00392, tetur95g00080Trypsin Inhibitor-like, cysteine rich domain protein0.0167YEDOG5_207753 (2)3.345.3*
tetur28g00360Beta-mannosidase0.0167YEA*OG5_129300 (4)5.544*
tetur06g00510Hypothetical protein0.0162YE3.941.1*
tetur55g00040Wannes-Thomas Secreted Protein (WTSP) 1b0.0131YETu_MCL_36 (13)1.816.4*
tetur14g02090Immunoglobulin E-set0.0128YE*OG5_133467 (14)9.1−2.7*
tetur07g01660Hypothetical protein0.0122YE5.110.5*
tetur28g01330Wannes-Thomas Secreted Protein (WTSP) 1a0.0119YETu_MCL_36 (13)3.737.1*
tetur31g00630Hypothetical protein with Armadillo-type fold0.0118YETu_MCL_25 (17)4.727.3*
tetur03g08710Hypothetical protein0.0111YED3.036*
tetur14g02080MD-2-related lipid-recognition domain protein0.0108YE*OG5_133467 (14)8.9−22.6*
tetur02g04310Glycoside hydrolase, catalytic core0.0100YE*OG5_127620 (2)7.9−1.8*
tetur516g00020Vitellogenin 1, partial0.0098NC*OG5_132251 (7)336.7−3.9*
tetur01g05230, tetur01g16473, tetur06g02570, tetur06g02580Cathepsin B0.0097YE*OG5_127800 (28)6.5−6.4*
tetur30g01440Serine protease0.0096YE*OG5_152337 (1)5.146.4*
tetur32g01880Hypothetical protein0.0094YETu_MCL_43 (12)2.662.3*
tetur28g01720Short-chain dehydrogenase/reductase SDR0.0091NC*OG5_128170 (22)4.9−46.1*
tetur14g02070MD-2-related lipid-recognition domain protein0.0091YE*OG5_133467 (14)7.8−16.7*
tetur10g00090Glycine-rich secreted protein0.0083YED5.031.5*
tetur09g04400*Cathepsin L0.0083YE*OG5_126607 (22)8.0−1.1
tetur43g00010*Vitellogenin 50.0082YE*OG5_132251 (7)536.3−7.5*
tetur01g01010Orphan Secreted protein0.0078YETu_MCL_212 (2)8.921.9*
tetur07g07380*Beta-galactosidase0.0074YM*OG5_128163 (4)5.5−2.3*
tetur10g00100Hypothetical protein0.0072YE4.224.4*
tetur04g09479Small Secreted Protein, Family F0.0068YETu_MCL_35 (14)5.965.8*
tetur01g00950Orphan secreted protein0.0068YEATu_MCL_211 (2)7.528.6*
tetur06g00230Hypothetical protein0.0061YEATu_MCL_25 (17)5.129.3*
tetur12g01860*Cathepsin L0.0057YE*OG5_126607 (22)8.2−1.5*
tetur31g00830Hypothetical protein0.0056YETu_MCL_25 (17)emb(0)14.4
tetur03g07920*Glutathione S-transferase, delta class0.0053NC*OG5_126942 (17)5.7−2.2*
tetur31g01040, tetur31g01060Hypothetical protein with Armadillo-type fold0.0051YETu_MCL_25 (17)8.647.7*
tetur03g08030Cathepsin B0.0051YE*OG5_127800 (28)13.3−15.8*
tetur16g03190Serine protease homologue0.0050YE*OG5_141111 (2)5.348.7*
tetur01g02510*Glutathione S-transferase, class delta0.0048NC*OG5_126942 (17)1.210.1*
tetur39g00810*Vitellogenin 20.0045YE*OG5_132251 (7)574.81.3*
tetur23g01640Flavin reductase0.0043NCOG5_131746 (2)2.71.3
tetur26g02320Superoxide dismutase, copper/zinc binding0.0043NC*OG5_127584 (8)1.83.2*
tetur19g01900Prolylcarboxypeptidase0.0043YE*OG5_130246 (1)3.5−1.8*
tetur18g03030*Alpha-2-macroglobulin0.0042NC*890.1−12.3*
tetur07g08034Serine proteases, trypsin family0.0042YE*OG5_149533 (13)1.04
tetur09g03880Serine protease0.0040YE*OG5_168371 (5)6.748.3*
tetur06g01060Cystatin0.0039YE*OG5_147492 (6)10.1−2.9*
tetur14g03160Hypothetical protein0.0039NETu_MCL_63 (8)emb(0)3.1
tetur06g01640Outer membrane lipoprotein Blc/Lipocalin0.0031YEOG5_130527 (50)3.8−2.3*
tetur20g01290Hypothetical protein0.0030YEOG5_126560 (8)9.3−12.2*
tetur13g03820Proteinase inhibitor I2, Kunitz metazoa0.0030YE*OG5_134456 (1)4.12.5*
tetur25g00650*Cathepsin L0.0030YE*OG5_126607 (22)16.4−1.4
tetur13g00600Hypothetical protein0.0029YE3.825.4*
tetur147g00020, tetur33g01260Glycoside hydrolase, subgroup, catalytic core0.0029YE*OG5_129423 (34)17.5−1
tetur01g00940Orphan secreted protein0.0029YEATu_MCL_212 (2)7.023.4*
tetur09g03620, tetur09g03650, tetur09g03670Cystatin0.0028YEOG5_147492 (6)7.0−1.8*
tetur22g00260Trypsin Inhibitor-like, cysteine rich domain protein0.0027YEOG5_176862 (1)11.32.6*
tetur02g12930Glycoside hydrolase, subgroup, catalytic core0.0027YE.R.*OG5_129423 (34)8.2−3.9*
tetur02g11340, tetur02g11390Aldo-keto reductase family B, member 10.0026NC*OG5_126583 (9)5.08.1*
tetur32g02327Short-chain dehydrogenase/reductase0.0022NC*OG5_128075 (3)emb(0)2.7*
tetur01g11910Chitinase0.0022YE*OG5_152454 (3)0.728.7*
tetur09g00900Hypothetical protein0.0022YE2.121.3*
tetur07g03440*, tetur109g00010Fructose-bisphosphate aldolase, class-I0.0018NC*OG5_127143 (3)2.02.8*
tetur03g09480*, tetur09g05350*, tetur09g05360Actin0.0017NCS*OG5_126595 (3)0.514.1*
tetur11g01500Carboxyl/cholinesterase0.0015YE*OG5_136350 (8)1.6−1.1
tetur03g04470Fibronectin, type III-like fold0.0012YETu_MCL_153 (3)6.71.3*
  • a accession number in the ORCAE database (http://bioinformatics.psb.ugent.be/orcae/overview/Tetur); an asterisk (*) indicates that the T. urticae protein has been found in faeces of mites (88) while a cross () indicates that the T. urticae protein was predicted with a transmembrane domain by TMHMM 2.0 (http://www.cbs.dtu.dk/services/TMHMM/).

  • b maximal rTop3 value across the four T. urticae host plant adapted lines (bean, soy, maize, tomato).

  • c presence (Y = yes, N = no) of a signal peptide as predicted by SignalP 4.0 (81).

  • d subcellular localization prediction by WoLF PSORT (CytoSkeleton/Cytoplast/Endoplasmic Reticulum/Extracellular/Mitochondrial) (82).

  • e gland expression localization determined by whole-mount in situ hybridization (ISH): Anterior or Dorsal podocephalic glands.

  • f homologues (BLASTp E-value threshold 1E-5) identified in the American house dust mite D. farinae (Df) (*).

  • g OrthoMCL group with the number of OrthoMCL members present in the T. urticae genome shown between brackets (see Table S7 for OrthoMCL details).

  • h overall average expression across all the feeding stages (larva, nymph, adult) relative to expression in the embryo stage (44); in case of gene groups the expression ratio of the first gene is shown; emb(0): no reads could be mapped to this gene in the embryo stage; (−) T. urticae gene was not annotated at time of experiment.

  • i fold change in gene expression between T. urticae proterosomas and intact T. urticae females (* = Benjamini-Hochberg corrected p value ≤ 0.05); in case of gene groups the FC of the first gene is shown; (−) no reads could be mapped to this gene.