Table VI Kd values for 2-oxohistidine peptides binding to S100A1
S100A1Oxidized peptidesUnoxidized peptidesFluorescent dyes
DyLight 550 Oxo-AGDyLight 550 Oxo-SEDyLight 650 Oxo-IADyLight 650 AGDyLight 650 SEDyLight 550 IADyLight 550DyLight 650
no calcium5.3E-9 ± 4.9E-9a,b5.2E-9 ± 2.1E-9a,b1.2E-8 ± 4.2E-9a,b3.9E-8 ± 4.0E-88.2E-8 ± 5.7E-83.1E-8 ± 2.0E-83.4E-7 ± 2.4E-73.6E-7 ± 3.6E-7
w/calcium7.3E-9 ± 4.8E-9a2.9E-9 ± 6.4E-10a2.8E-8 ± 9.8E-9a1.3E-7 ± 9.1E-88.5E-8 ± 5.5E-86.6E-8 ± 3.7E-85.6E-8 ± 3.8E-81.2E-7 ± 8.4E-8
  • a Significant difference to its normal peptide control and fluorescent dye control (p < 0.05, unpaired t-test).

  • b No significant difference compared to calcium group (p > 0.1, unpaired t-test).