Table III Kd values for 2-oxohistidine peptides binding to identified proteins. All Kd values were determined by dose-response measurements. Three pairs (oxidized vs. nonoxidized) of peptide probes with different sequences were investigated
NameOxidized peptidesUnoxidized peptidesFluorescent dyes
DyLight 550 Oxo-AGDyLight 550 Oxo-SEDyLight 650 Oxo-IADyLight 650 AGDyLight 650 SEDyLight 550 IADyLight 550DyLight 650
thrS1.2E-8 ± 9.6E-10a6.7E-9 ± 1.9E-9a3.9E-8 ± 4.7E-9a1.4E-7 ± 3.5E-82.5E-7 ± 8.3E-81.0E-7 ± 6.8E-88.5E-8 ± 1.1E-81.1E-7 ± 1.6E-8
yqjG1.1E-8 ± 9.2E-10a3.4E-9 ± 2.4E-10a1.4E-8 ± 1.7E-9a4.9E-7 ± 2.1E-71.0E-7 ± 4.5E-91.2E-7 ± 7.0E-82.9E-7 ± 9.7E-81.4E-7 ± 2.7E-8
yajL5.6E-8 ± 2.8E-8a1.4E-8 ± 7.5E-9a1.0E-7 ± 3.1E-8a3.7E-7 ± 1.9E-78.6E-7 ± 2.8E-72.2E-7 ± 1.2E-72.5E-7 ± 1.3E-74.4E-7 ± 2.9E-7
hemE8.7E-10 ± 6.1E-11a6.9E-10 ± 2.3E-11a5.6E-9 ± 5.3E-10a1.9E-7 ± 3.4E-81.2E-8 ± 7.7E-101.2E-7 ± 3.5E-81.4E-7 ± 5.4E-84.8E-8 ± 5.7E-9
ilvA1.6E-8 ± 4.1E-9a2.8E-8 ± 3.6E-8a5.9E-7 ± 1.7E-7a2.9E-7 ± 4.1E-82.9E-7 ± 5.9E-81.5E-6 ± 6.8E-78.5E-8 ± 5.4E-81.3E-6 ± 5.3E-7
prpD1.6E-7 ± 1.7E-7a9.0E-8 ± 1.8E-7a1.3E-7 ± 2.1E-8a7.3E-7 ± 1.0E-76.2E-7 ± 4.2E-77.9E-7 ± 1.7E-71.3E-6 ± 8.7E-72.4E-6 ± 5.7E-7
zwf1.4E-8 ± 1.5E-9a2.3E-8 ± 2.7E-8a7.2E-8 ± 1.7E-8a1.2E-6 ± 5.3E-75.8E-7 ± 2.2E-73.7E-7 ± 2.7E-75.6E-7 ± 3.6E-76.4E-7 ± 2.7E-7
eda3.9E-9 ± 2.7E-10a2.0E-9 ± 2.6E-10a9.2E-8 ± 1.6E-8a3.3E-7 ± 1.1E-72.6E-7 ± 9.4E-82.6E-7 ± 1.1E-72.2E-7 ± 2.4E-74.6E-7 ± 2.1E-7
gor2.4E-8 ± 3.5E-9a1.2E-8 ± 1.8E-9a8.3E-8 ± 3.4E-8a1.1E-6 ± 5.5E-78.6E-7 ± 5.0E-72.2E-7 ± 8.2E-89.0E-7 ± 5.0E-76.1E-7 ± 3.7E-7
pqqL5.4E-9 ± 4.7E-10a1.8E-9 ± 2.3E-10a6.7E-8 ± 1.1E8a3.0E-7 ± 2.0E-73.0E-7 ± 1.1E-73.7E-7 ± 2.7E-71.5E-7 ± 9.2E-86.3E-7 ± 3.8E-8
  • a Significant difference to its normal peptide control and fluorescent dye control (p < 0.05, unpaired t-test).