Table II Significantly altered proteins after the incubation of HT-29 cells with GI50, TGI, and LC50 concentrations of CA and CS for 24 hours
NameCACSNameCACSNameCACS
GI50TGILC50GI50TGILC50GI50TGILC50GI50TGILC50GI50TGILC50GI50TGILC50
Antioxidant defenceUnfolded Protein Response (tRNA charging and AAs metabol.)Chromatine
    GCLCa0.921.231.29    AARScn.s.0.620.96n.s.n.s.    HMGN2n.s.n.s.−1.16n.s.
    GCLMa1.371.53n.s.1.111.54    WARSc0.88n.s.1.15n.s.    HIST1H2AJn.s.n.s.n.s.−1.42n.s.
    TXNRD1a1.081.411.410.851.131.28    GARSc0.630.991.21n.s.n.s.n.s.    CBX3n.s.n.s.n.s.n.s.n.s.−0.61
    TXN10.61n.s.n.s.n.s.n.s.n.s.    SARScn.s.0.941.17Other functions
    SRXN1a2.63    YARScn.s.0.60n.s.    TMEM109n.s.n.s.−0.71n.s.n.s.n.s.
    MT2Aan.s.n.s.1.48n.s.n.s.1.04    SLC3A2c0.821.491.78n.s.0.721.02    GLG1n.s.−0.90n.s.n.s.n.s.
NADPH generation    SLC1A5cn.s.n.s.n.s.n.s.0.59n.s.    TMPOn.s.n.s.−0.65n.s.n.s.n.s.
    SLC2A1n.s.0.94n.s.n.s.n.s.n.s.    ASNSc1.632.471.62    KPNA2n.s.−0.83−1.19n.s.n.s.n.s.
    ME1a0.850.910.94n.s.0.91    PSAT1c0.590.981.09n.s.0.80    STMN1n.s.−0.65−0.78n.s.n.s.n.s.
    IDH1an.s.n.s.0.75n.s.n.s.n.s.E2F-targets    GMDS−0.60n.s.n.s.
    PGDa0.690.770.79n.s.n.s.n.s.    RRM1dn.s.−0.88n.s.n.s.n.s.    HN1Ln.s.n.s.n.s.n.s.−0.69
    G6PDan.s.0.68n.s.n.s.n.s.n.s.    COPS8dn.s.−0.71n.s.n.s.n.s.n.s.    RAB1An.s.n.s.n.s.−0.97
Detoxification metabolism    TOP2Adn.s.−1.33−2.46n.s.n.s.n.s.    SLC12A2n.s.−1.87
    AKR1B10a1.521.821.611.371.58n.s.    RBBP4dn.s.n.s.−0.85n.s.n.s.n.s.    ALDH18A1n.s.n.s.−0.59n.s.−0.66
    AKR1C11.832.242.941.79n.s.1.30    CSDE1dn.s.n.s.−0.66n.s.n.s.n.s.    S100Pn.s.n.s.1.40n.s.n.s.n.s.
    AKR1C31.261.371.31n.s.n.s.0.71Pyrimidine and purine nucleotides biosynthesis    GFPT1n.s.n.s.1.06n.s.n.s.n.s.
Ubiquitin-proteasomal degradation    CADn.s.n.s.−0.64n.s.−0.67n.s.    TOP1cn.s.n.s.0.60n.s.n.s.n.s.
    PSMC1an.s.n.s.n.s.n.s.0.730.76    GMPSn.s.n.s.−0.61n.s.n.s.n.s.    LRRC59n.s.n.s.0.59n.s.n.s.n.s.
    PSMC2an.s.n.s.n.s.n.s.0.670.72    HPRT1n.s.n.s.−0.61n.s.    ANXA5n.s.n.s.0.62n.s.n.s.n.s.
    PSMC3an.s.n.s.n.s.n.s.0.72n.s.DNA replication/cell proliferation    GOT1n.s.0.74n.s.n.s.n.s.
    PSMC5an.s.n.s.n.s.0.87    NASPn.s.n.s.−0.80n.s.n.s.n.s.    RCN10.66n.s.n.s.
    PSMA4an.s.n.s.n.s.n.s.n.s.0.69    NAP1L1n.s.n.s.−0.60n.s.n.s.n.s.    CAPN20.62n.s.
    PSMA7an.s.n.s.n.s.n.s.0.63n.s.    MKI67n.s.−1.32n.s.n.s.−1.07    CLIC40.72
    PSMD1an.s.n.s.n.s.0.62    SERPINB50.60n.s.    UGDHa0.971.061.06n.s.n.s.n.s.
    PSMD2an.s.0.75Cellular adhesion    SERPINH1n.s.0.87
    VCPn.s.n.s.n.s.n.s.n.s.0.64    GCNT31.79    ACSL5n.s.0.83
Chaperones    CD44n.s.n.s.n.s.n.s.n.s.−0.92    CD59n.s.n.s.n.s.n.s.n.s.0.80
    HSP90AA1n.s.n.s.n.s.n.s.0.780.81    MUC5ACn.s.n.s.n.s.n.s.n.s.−0.69    CALB2n.s.n.s.n.s.0.70n.s.n.s.
    HSPH1n.s.n.s.n.s.0.870.961.33    CNN2n.s.−1.04−1.40n.s.n.s.−1.05    S100A14n.s.n.s.n.s.n.s.0.82n.s.
    HSPA1An.s.n.s.0.681.181.482.29    POF1Bn.s.−0.96−1.65n.s.−0.96    C1QBPn.s.n.s.n.s.0.61n.s.
    SQSTM11.421.782.521.591.741.77Cytoskeleton    NAMPT0.651.101.41n.s.0.930.96
Unfolded Protein Response (ERAD)    SLC9A3R1n.s.n.s.−0.69n.s.n.s.−0.59    SFN0.610.641.39n.s.1.071.23
    SEC61Bbn.s.n.s.0.83n.s.n.s.    MSNn.s.n.s.n.s.n.s.0.64n.s.    ANXA10.811.071.52n.s.n.s.0.79
    HYOU1bn.s.0.591.21n.s.n.s.n.s.    VIL1n.s.n.s.n.s.n.s.−0.73n.s.    PSAP0.73n.s.1.19n.s.n.s.1.27
    SSR1bn.s.n.s.0.65n.s.    TUBA1Cn.s.n.s.n.s.n.s.0.59    RAB10n.s.n.s.0.64n.s.0.84
    SSR4bn.s.n.s.0.64n.s.
    HSPA5b,cn.s.0.651.21n.s.n.s.0.59
    HSP90B1b,cn.s.n.s.0.65n.s.n.s.n.s.
    HSPA9bn.s.n.s.0.59n.s.n.s.n.s.
  • Proteins whose expression is regulated by: aNrf2;

  • b XBP1;

  • c ATF4;

  • d E2F1. n.s., not significant.