Table III Different experimental approaches carried out for the identification of the proteins listed in Table I and II and post-translational modifications characterized

A, high resolution MS/MS of the intact protein; B, coincidence with standards; C, N-terminal Edman sequencing; D, peptide mass fingerprint (trypsin) by MALDI-TOF-MS and/or by ESI-MS; E, MS/MS of the trypsin digest; F, cysteine alkylation by vinyl-pyridine; G, removal of N-terminal acetyl group; H, phosphatase treatment.

IdentityN-term lossN-term acetyl.OtherExp appr.Mav exp.aMav theor.bMav theor.c
1Thymosin β10MYesA,B4936.54936.54936.5
2Thymosin β4MYesA,B4963.54963.54963.5
6S100A8-NoA108341083410834
8Stefin A-NoA,D110061100610875
10Stefin B-YesA111821118211182
11S100A7 (D27 var.)MYesA113681136811326
12S100A7 (E27 var.)MYesA113821138211382
13Antileukoproteinase-No8 S-SA,C117101171011710
14S100A9MTCKMYesA,D,E1268912689-
15S100A9MTCKMYesPhosG,H1276912769-
16S100A9MYesA,D,E131531315313111
17S100A9MYesPhosG,H132331323313191
20Lysozyme-No4 S-SC,F146941469414694
23SPRR3 (17 kDa)MYes7 cysD,E,F,G172391723917328
SPRR3 (18 kDa)MYes8 cysD,E180651806518154
24Histone H1c???D22365?22260
28S100A12MNoA104441044410444
29S100A11MYesA116511165111740
38Peroxiredoxin 6 (?)---249042490424904
40α-globinM-B,D,E151281512815128
41γ-globinsdM-B,D,E160091600916009
  • a Experimental error of the average mass: ± 0.02%.

  • b Theoretical average mass computed considering the PTMs reported in the table.

  • c Theoretical average mass available in Swiss-Prot data-bank (in italics when the theoretical Mav reported in Swiss-Prot did not correspond to the experimental).

  • d Only the average mass of Aγ globin is reported. The Mav of Gγ globin is 15995 Da.