Antibodies used in this study. mAb1 is a monoclonal antibody raised against a peptide. mAb2 through mAb5 are monoclonal antibodies with epitopes previously determined in the literature. All of the polyclonal antibodies were raised against peptides selected from the random sequence peptide array
| Name | Immunogen | Clonality | Isotype | Clone name | Sequence | pI | Hydropathicity |
|---|---|---|---|---|---|---|---|
| mAb1 | HA peptide | Monoclonal | IgG1 | 16B12 | YPYDVPDYA | 3.56 | −0.9 |
| mAb2 | Human IL2 | Monoclonal | IGg1 | LNKB2 | KPLEEVLNL | 4.53 | −0.044 |
| mAb3 | Human p53 | Monoclonal | IgG1 | PAb240 | RHSVV | 9.76 | −0.02 |
| mAb4 | Human p53 | Monoclonal | IgG2b, IgG2a | DO-7, BP53–12 | SDLWKL | 5.55 | −0.25 |
| mAb5 | Human Tubulin-alpha | Monoclonal | IgG1kappa | DM1A | AALEKD | 4.67 | −0.583 |
| pAb1 | KLH-peptide | Polyclonal | IgG detected | NA | MDQDDGEGVIGHFHPILGSC | 4.21 | −0.185 |
| pAb2 | KLH-peptide | Polyclonal | IgG detected | NA | EFWDKEWHTRADWPVWDGSC | 4.43 | −1.245 |
| pAb3 | KLH-peptide | Polyclonal | IgG detected | NA | TIPAHNIFWILYFSIGTGSC | 6.4 | 0.87 |
| pAb4 | KLH-peptide | Polyclonal | IgG detected | NA | PAMKHREPHWVIPGIIWGSC | 8.64 | −0.13 |
| pAb5 | KLH-peptide | Polyclonal | IgG detected | NA | EFSNPTAQVFPDFWMSDGSC | 3.49 | −0.315 |