Table I Peptides identified by MS/MS in enriched peptide pools using anti-AMTR (A) and anti-LGYR antibodies (B)
m/zC terminusPeptide sequenceProteinIDNon-treatedSB216763-treated
Parent ion detectedMascot scoreDistance to next hitIdentity thresholdParent ions detected in replicatesParent ion detectedMascot scoreDistance to next hitIdentity thresholdParent ions detected in replicates
A
    1268.7AMTRHSHAVSTAAMTRHost cell factorHCFC1Yes251125Yes
    4727.4AMTRGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRaCatenin β-1CTNB1Yes434014Yes
    AA position 2 Met ⇆ Ser
        1048.6ASTRGPILSFASTRReplication factor C subunit 5RFC5Yes362825YesYes433725Yes
        1559.8ASTRNSHPPANLPTPASTRUncharacterized protein C10orf12CJ012Yes574724Yes
        1634.8ASTRTQMLDQEELLASTRKinesin heavy chainKINHYes554124Yes
        1736.9ASTRASDQLQVGVEFEASTRMitochondrial import receptor subunit TOM40 homologTOM40Yes483623YesYes847223Yes
        2408.3ASTRLEPHKPQQIPIDSTVSFGASTRNuclear inhibitor of protein phosphatase 1PP1R8YesbYesYes14712720Yes
    AA position 2 Met ⇆ Thr
        894.5ATTRSFVIATTRVacuolar protein sorting-associated protein 18VPS18Yes321525Yes
        1390.7ATTRNGTWLQATAATTRWD repeat protein 85WDR85Yes37824Yes
        1691.9ATTRVQGSDSDEEVVVATTRValyl-tRNA synthetaseSYVYes422724YesYes1088524Yes
        2172.1ATTRSNGWILPTVYQGMYNATTRaAflatoxin B1 aldehyde reductase member 2ARK72YesbNDcYes22722Yes
        2336.3ATTRSPSDPSHVSVPPPPLLLPAATTRPolyhomeotic-like protein 3PHC3Yes463721Yes
    AA position 2 Met ⇆ Val
        840.5AVTRAPIIAVTRPyruvate kinase isozymes M1/M2KPYMYesbYesYes411219Yes
        1422.7AVTRCGEMAQAASAAVTRValyl-tRNA synthetaseSYVYes431724Yes
    AA position 2
        1191.6AATRLAPEYEAAATRProtein-disulfide isomerase A3 precursorPDIA3YesbYesYes835926Yes
        1162.6AQTRNLCYHAQTRE3 ubiquitin-protein ligase HUWE1HUWE1YesbNDYes341926Yes
        1108.5AHTRDCGEYAHTR28 S ribosomal protein S2, mitochondrialRT02Yes544025Yes
        1247.7ANTRLFEVGGSPANTRSerine/threonine-protein phosphatase 2B catalyticPP2BAYesbYesYes826025Yes
        1499.8AYTRTEPELLVAHAYTRHeme oxygenase 1HMOX1YesbNDYes392923Yes
        1832.9AGTRSQLGAHHTTPVGDGAAGTRTransmembrane protein 41BTM41BYesbYesYes766423Yes
B
    805.6LGYRIAILGYRGTP-binding protein RhebRHEBYes251717Yes
    928.5LGYREQYLGYRNucleoporin p58/p45NUPL1Yes271026YesYes483026Yes
    951.6LGYRTLDNLGYRγ-AdducinADDGYes251124YesYesbYes
    1026.6LGYRYLELLGYRProtein transport protein Sec31ASC31AYes472024YesYes543524Yes
    1076.6LGYRLNIGGGGLGYRATP-dependent RNA helicase DDX42DDX42YesbYesYes402025Yes
    1183.6LGYRVDVFTNLGYRTHO complex subunit 6 homologTHOC6Yes331325Yes
    1552.8LGYRVAAQQGFDLDLGYRZinc finger MIZ domain-containing protein 1ZMIZ1Yes462724Yes
    2804.3LGYRTEPMAWNETADLGLDIGAQGEPLGYRaCatenin β-1CTNB1Yes23222018Yes
    AA position 3 Tyr ⇆ Phe
        1599.9LGFRQPELPEVIAMLGFR (methionine oxidized)Catenin δ-1CTND1YesbYesYes413223Yes
        2633.2LGFRQVQHEESTEGEADHSGYAGELGFRdUbiquitin fusion degradation protein 1 homologUFD1Yes847721YesYes835921Yes
        3381.6LGFRDIEQVPQQPTYVQALFDFDPQEDGELGFRGrowth factor receptor-bound protein 2GRB2Yes341418YesYes533118Yes
    AA position 3 Tyr ⇆ Leu
        932.6LGLRFVDILGLRβ-Catenin-like protein 1CTBL1Yes342119YesYesbYes
        1291.8LGLRAAVLKPVLLGLR + acetyl (N terminus)Programmed cell death protein 2-likePDD2LYes271820Yes
        1698.8LGLRAWDQEAEGAGPELGLRAldehyde dehydrogenase family 16 member A1A16A1Yes432623Yes
    AA position 3 Tyr ⇆ Ala
        1742.0LGARFGTVLTEHVAAAELGARDeoxyribonucleoside 5′-monophosphate N-glycosidaseRCLYes302721YesYesbYes
        1781.9LGARLAEMPADSGYPAYLGARaV-type proton ATPase catalytic subunit AVATAYes756024YesYes816924Yes
    AA Position 3 Tyr ⇆ Gly
        1409.8LGGRVLATTFDPYLGGRHeat shock 70-kDa protein 4HS74LYes241023Yes
    AA position 1 Leu ⇆ Ile
        2000.0IGYRFLYGENMPPQDAEIGYRDual specificity protein kinase TTKTTKYes412923Yes
  • a Also identified containing oxidized methionine.

  • b Poor fragmentation.

  • c ND, not detected.

  • d Also identified with N-terminal pyroglutamate.