Table I Bioinformatic Src and p38 MAPK phosphorylation motif predictions. Identified phosphopeptides were submitted to GPS 2.1 to predict potential p38 MAPK and Src Tyr kinase motifs. Proteins were grouped by molecular function and the NCBI accession number, the phosphopeptide identified in the proteomic analysis, and the phosphorylated residue(s) listed. A raised numeral indicates the amino acid-specific phosphorylation of serine, threonine, or tyrosine. Phosphorylated amino acid(s) predicted to comprise a Src family phosphorylation motif are denoted by Y. Those comprising a potential p38 MAPK phosphorylation motif are denoted by S and T
Identified ProteinAccession NumberPeptide sequencePhosphorylated Residue
Adhesion
Plakophilin 2 isoform 2b4758932LEIS151PDS154SPERS151, S154
Cell cycle
    Retinoblastoma-like protein 1 isoform b34577077EKEAVIT385PVASATQSVS395RT385
Cytoskeletal regulation
    Drebrin 1 isoform b18426913S339PS341DSST345AS347T348PVAEQIERS339, T348
GTPases and GTPase-binding proteins
    ArfGAP with SH3 domain, ankyrin repeat and PH domain 319923540S862PEDGPSARS862
    Exophilin 5 (Slac2-b)21359818TSCDSLDLSSAALPDS887SPS890KS887
    Ras protein-specific guanine nucleotide-releasing factor 1 isoform24797099S232PHADSMRS232
Immune/inflammatory response
    Lactotransferrin; transferrin4505043PVAAEVY102GT104ERY102
Ion/Metabolite Transport
    Barttin17017967S91PSPQPPY98VRS91, Y98
Kinase/Phosphatase
    G protein coupled receptor kinase 555958649GESMALNEKQILEKVNSQFVVNLAY251A-Y253ETKY251
    Serine/Threonine kinase SMG-1 isoform 214719394SSCT2305T2306PDEWWT2306
    PREDICTED: similar to protein phosphatase 2A 48 kDa regulatory51472904KT75GPRACSTPLSTSS88PS90PRS88, S90
Miscellaneous
    Arsenate resistance protein 233383231DEHSS89DPY92Y92
    T-complex protein 1 subunit theta42662316IGLS124VSEVIEGY132EIACRKY132
Receptor/signal transduction
    Aryl hydrocarbon receptor4502003SS90PTERNGGQDNCS90
    Chemokine (C-X-C motif) receptor 4 isoform b4503175T13EEMGSGDY21Y21
    G-protein coupled receptor 8811545753DGAGGSY112Y112
    Olfactory receptor, family 6, subfamily C, member 7641201775PLHY129TTIMS134DRY129
Redox
    Cytochrome b5 reductase b5R.2 isoform 147778923AYT70PVSSDDDRT70
Secreted
    Angiogenin, RNase A family, 5 precursor4557313GLTS61PCKS61
Structural proteins
    Fibronectin 1 isoform 3 preproprotein16933542GDS1527PASS1531KS1527
    Usherin isoform B46249406S442NFTPY447SRGNVT453FY447
Translation/transcription
    Protein CASP31652240Y493SKAMQEAGSTSMIFSTGPYY493
Ubiquitination
    E3 SUMO-protein ligase RanBP2638207VT2027GEEDEKVLY2036Y2036
Unknown Function
    AlkB, alkylation repair homolog 540353210Y60QEDSDPERY60
    Hypothetical protein LOC22010831542760SSS459DLS462VVS465AY467Y467
    Hypothetical protein LOC34848732699058CFQTTNGY67LSDS71RY67
    Nucleoporin 214kDa33946327HGAPS1081PS1083HPISAPQAAAAAALRS1081
    PREDICTED: NHS-like 1 isoform 151465299SRTPPSAASSRY1511SMRY1511
    Testis expressed sequence 1513775174LINVCGDS1842PKS1842
    Tetratricopeptide repeat domain 377662078IKLSDY366DSS369EEAIRT375LY366