Table II Cysteine oxidative modifications of UCH-L1 in the hippocampus of control and stress-treated mice

Peptides with the highest ions score for each spot were tabulated. Modified residues were highlighted in bold and italic. ND, modified peptides were not detected. MS/MS spectra of modified peptides are presented in supplementary Fig. S7.

ResidueModificationStart-EndMass (m/z) ExperimentalMass TheoreticalDelta Mass (Da)ScoreSequence
ControlStress
spot 3spot 2spot 1spot 4spot 3spot 2spot 1
C90Dioxidation84–115851.66683402.6725−0.034454109ND61104NDNDQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLK
Trioxidation1140.56273418.6674−0.001215616359127156151ND
Cys-SO2-SH1145.88493434.6446−0.0117103611510612686ND
C152Trioxidation136–153987.93051973.8497−0.0033713623696842NDNEAIQAAHDSVAQEGQCR
Cys-SO2-SH995.92101989.82690.00067455ND6446NDND