Table I Proteins differentially changed because of chronic restraint stress in the chronic restraint stress (CRS) mouse model, as revealed by proteomic studies

Each of 63 protein spots from Fig. 1B was identified using nanoUPLC-ESI-q-TOF tandem mass spectrometry, classified based on their biological process.

Spot #Accession #Uniprot IDProtein nameplMassMascot Score# of Distinct Peptides% CoverageRatio (CRS/Control)p value
9D14D
Protein metabolic process
    1Q9CZC8SCRN1Secernin-14.67462973341636−2.00−2.215.000E-03
    2Q9D1A2CNDP2Cytosolic non-specific dipeptidase5.43527345822652−1.591.211.915E-04
    3Q9D1A2CNDP2Cytosolic non-specific dipeptidase5.43527344691332−2.521.211.000E-03
    30Q9D1A2CNDP2Cytosolic non-specific dipeptidase5.435273449421561.381.463.700E-02
    19P63242IF5A1Eukaryotic translation initiation factor 5A-15.071682140222−2.81−2.503.903E-05
    63P63242IF5A1Eukaryotic translation initiation factor 5A-15.0716821643304.703.117.108E-07
    24P63038CH6060 kDa heat shock protein, mitochondrial5.916091717341.921.324.141E-06
    34Q8BG32PSD1126S proteasome non-ATPase regulatory subunit 116.084740759323471.311.216.581E-05
    43Q7TQI3OTUB1Ubiquitin thioesterase OTUB14.85312501278381.311.792.108E-04
    57P61087UBE2KUbiquitin-conjugating enzyme E2 K5.33223931215321.491.282.100E-02
Carbohydrate metabolic process
    29P62814VATB2V-type proton ATPase subunit B, brain isoform5.5756515106944723.994.702.000E-03
    45P16125LDHBl-lactate dehydrogenase B chain5.73654943218412.312.511.718E-05
    53Q9DBJ1PGAM1Phosphoglycerate mutase 16.672881442716681.321.291.454E-05
    56P17751TPISTriosephosphate isomerase5.563217127613452.112.333.024E-04
Vitamin metabolic process
    44Q8K183PDXKPyridoxal kinase5.883499348822494.213.021.222E-05
Generation of precursor metabolites and energy
    21Q9D3D9ATPDATP synthase subunit delta, mitochondrial4.981758928232−1.19−1.321.200E-02
    55Q9DCT2NDUS3NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial6.673013133211442.011.895.183E-05
Haloacid dehalogenase (HAD)-type phosphatases
    9Q3UGR5HDHD2Haloacid dehalogenase-like hydrolase domain-containing protein 25.7287301201361−2.39−3.213.528E-05
    52Q3UGR5HDHD2Haloacid dehalogenase-like hydrolase domain-containing protein 25.72873016415523.113.306.398E-09
    46Q8CHP8PGPPhosphoglycolate phosphatase5.2345192227331.80−1.573.372E-04
Intracellular protein transport, vesicle mediated transport
    7P47754CAZA2F-actin-capping protein subunit alpha-25.57329473521550−1.92−2.215.197E-06
    48P47754CAZA2F-actin-capping protein subunit alpha-25.573294751321582.713.331.339E-07
    31Q61598GDIBRab GDP dissociation inhibitor beta5.935050542625501.991.315.000E-03
    32Q61598GDIBRab GDP dissociation inhibitor beta5.9350505133665702.711.703.291E-05
    49P28663SNABBeta-soluble NSF attachment protein5.323353564723601.431.233.920E-05
Neuronal migration, cell motility
    12Q61206PA1B2Platelet-activating factor acetylhydrolase IB subunit beta5.57255652441035−1.41−1.097.353E-04
Neural differentiation
    5P18872GNAOGuanine nucleotide-binding protein G(o) subunit alpha5.34400596162836−3.21−1.796.652E-06
    6P18872GNAOGuanine nucleotide-binding protein G(o) subunit alpha5.34400594022249−3.41−1.236.070E-04
    41P18872GNAOGuanine nucleotide-binding protein G(o) subunit alpha5.344005963123432.882.018.003E-05
    42P63085MK01Mitogen-activated protein kinase 16.54124936318442.222.491.507E-05
    47P62874GBB1Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-15.63735357024551.721.988.760E-07
Synaptic plasticity, neurite outgrowth, neuronal morphology
    18Q9CPW4ARPC5Actin-related protein 2/3 complex subunit 55.4716278271937−2.70−2.721.395E-04
    62Q9CPW4ARPC5Actin-related protein 2/3 complex subunit 55.47162782358262.792.113.627E-04
    20Q63810CANB1Calcineurin subunit B type 14.64192881361365−1.49−1.101.300E-02
    25O08553DPYL2Dihydropyrimidinase-related protein 2 (CRMP2)5.956223947442.354.029.666E-07
    26O08553DPYL2Dihydropyrimidinase-related protein 2 (CRMP2)5.956223957221.702.418.359E-07
    27O89053COR1ACoronin-1A6.055095756422523.222.284.698E-06
    33Q9R111GUADGuanine deaminase5.365098171127611.771.292.445E-06
    35P60710ACTBActin, cytoplasmic 15.2941710116038672.832.414.293E-08
    37P60710ACTBActin, cytoplasmic 15.2941710178764713.122.772.218E-04
    36P63260ACTGActin, cytoplasmic 25.3141766179570732.772.504.822E-05
    39P61161ARP2Actin-related protein 26.29447322048212.222.593.618E-07
    40P61161ARP2Actin-related protein 26.294473248617321.891.474.540E-02
    61Q9QXT0CNPY2Protein canopy homolog 24.9520754462161.811.554.251E-04
Calcium dependent regulation of neural plasticity
    4Q04447KCRBCreatine kinase B-type5.44268616455870−4.211.021.118E-04
    17P84075HPCANeuron-specific calcium-binding protein hippocalcin4.87224131941555−1.51−1.301.861E-04
    51P63328PP2BASerine/threonine-protein phosphatase 2B catalytic subunit alpha isoform5.585860638117372.812.717.667E-04
    60Q91X97NCALDNeurocalcin-delta5.23222311937241.77−1.023.500E-02
Protein aggregation, neurodegenerative disease-related
    11Q9R0P9UCHL1Ubiquitin carboxyl-terminal hydrolase isozyme L15.14248222801130−2.02−1.752.970E-02
    54Q9R0P9UCHL1Ubiquitin carboxyl-terminal hydrolase isozyme L15.142482238414433.631.761.104E-06
    15Q91ZZ3SYUBBeta-synuclein4.3814043141329−1.91−1.423.148E-07
    16O55042SYUAAlpha-synuclein4.74144762841044−1.19−1.472.695E-04
    38Q9Z2Q6SEPT5Septin-56.214272152616271.321.304.286E-06
Reactive oxygen species generation
    8O54983CRYMKetimine reductase mu-crystallin5.44335028693154−1.30−1.191.260E-05
    14Q61171PRDX2Peroxiredoxin-25.22176598718−2.70−4.219.768E-07
    59Q61171PRDX2Peroxiredoxin-25.22176565828661.691.881.859E-04
    28O08749DLDHDihydrolipoyl dehydrogenase, mitochondrial7.995423854225463.691.192.300E-04
Extracellular antioxidant defenses
    22P07724ALBUSerum albumin5.7568648187792.711.991.532E-08
    23P07724ALBUSerum albumin5.7568648105591.791.421.966E-06
Anti-apoptotic role
    10P682541433T14-3-3 protein theta4.69277615313162−2.53−2.331.348E-07
    13P63028TCTPTranslationally-controlled tumor protein4.76194503371437−1.21−1.421.638E-04
    58P63028TCTPTranslationally-controlled tumor protein4.76194501666302.121.592.000E-03
    50P622591433E14-3-3 protein epsilon4.6329155117640611.721.613.598E-04