Table III Post-translational modifications of UCH-L1 in the hippocampus of control and stress-treated mice

Peptides with the highest ions score for each spot were tabulated. Modified residues were highlighted in bold and italic. ND: Modified peptides were not detected, N, Q: Deamidated, deamidated; M, oxidation. MS/MS spectra of modified peptides are presented in supplementary Fig. S7.

ModificationResidueStart-EndMass (m/z) ExperimentalMass TheoreticalDelta Mass (Da)Score (total mascot score)Sequence
ControlStress
spot 3 (923)spot 2 (1241)spot 1 (694)spot 4 (505)spot 3 (1055)spot 2 (658)spot 1 (394)
PhosphorylationT85/C90_trioxi84–1151167.21833498.6338−0.000742162ND156112NDNDQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLK
S119116–129879.89761757.7873−0.006677637376687075QFLSETEKLSPEDR
S188179–1991155.99772309.9875−0.0067685862586258NDMPFPVNHGASSEDSLLQDAAK
AcetylationK7166–78764.40471526.78280.01214232ND254131NDQIEELKGQEVSPK
K123116–129574.28601719.83150.00473016ND1611NDNDQFLSETEKLSPEDR