Table I Comparison of the sample preparation protocols for tomato phosphoproteomics
ProtocolAmountLysis bufferDepletion methodDigestion buffer
Lysis efficiency200 μg100 mm Tris-HClN/A100 mm Tris-HCl
200 μg12 mm SDC-SLSN/A2.4 mm SDC-SLS
200 μg6 m Gdn-HClN/A600 mm Gdn-HCl
Interferences depletion200 μg6 m Gdn-HCl10K Daa50 mm ABC
200 μg6 m Gdn-HClM.-C. precipitationb1 m Urea
Digestion buffer200 μg6 m Gdn-HClM.-C. precipitation1 m Urea
200 μg6 m Gdn-HClM.-C. precipitation2.4 mm SDC-SLS
  • a Molecular weight cut-off filter.

  • b Methanol-chloroform precipitation.