Table I Oligonucleotide sequences used for CRISPR-Cas9 genome editing experiments
Oligonucleotide sequences for altMiD51 knock outOligonucleotide sequences for MiD51 knock out
Genomic target site5′-TGGAGCCGAGAGGCGGTGCT-3′5′-CGCTGGCAGTTAAGCGGGTA-3′
Top oligonucleotide5′-CACCGAGCACCGCCTCTCGGCTCCA-3′5′-CACCGTACCCGCTTAACTGCCAGCG-3′
Bottom oligonucleotide5′-AAACTGGAGCCGAGAGGCGGTGCTC-3′5′-AAACCGCTGGCAGTTAAGCGGGTAC-3′
T7 endonuclease 1 mismatch assays primers5′-GGGGTCTCTGGAACTTGGAT-3′ 5′-TCCTTTTCTCGGTCCCTTGC-3′5′-GGTCCCAGTACTTATGGCCG-3′ 5′-CCACGCAGAAAATCTCAGGG-3′