Table I AP and BG peptides and transitions selected for LC-MRM MS
ProteinPeptideaPrecursor m/zProduct m/z
β-galactosidase (BG)LPSEFDLSAFLR (BG 698)697.9593.34, 706.42, 821.45, 968.52
LPSEFDLSAFL*R702.9603.35, 716.43, 831.46, 978.53
LWSAEIPNLYR (BG 681)681.4662.36, 775.45, 904.49, 1062.56
LWSAEIPNLY*R686.4672.37, 785.45, 914.50, 1072.57
APLDNDIGVSEATR (BG 729)729.4563.28, 719.37, 832.45, 1061.52
APLDNDIGVSEAT*R734.4573.29, 729.38, 842.46, 1071.53
Alkaline phosphatase (AP)AAQGDITAPGGAR (AP 593)592.8457.25, 528.29, 629.34, 914.47
AAQGDITAPGGA*R597.8467.26, 538.30, 639.34, 924.48
APGLTQALNTK (AP 557)557.3546.32, 674.38, 775.43, 945.54
APGLTQALNT*K561.3554.34, 682.40, 783.44, 953.55
NYAEGAGGFFK (AP 581)580.8555.29, 683.35, 812.39, 883.43
NYAEGAGGFF*K584.8563.31, 691.36, 820.41, 891.44
  • a Peptides marked with * are isotopically labeled.