TABLE I

Composition of each of the standard protein mixtures used for the experiment shown in Fig. 5

After separately labeling with the indicated form of ICAT™ reagent the two mixtures were combined and concurrently separated by 2DE. The expected quantitative ratios, the quantitative ratios that were experimentally determined, and the calculated error are also displayed.

ProteinMixture 1 (d0-labeled)Mixture 2 (d8-labeled)Expected ratioObserved ratioError
μgd0:d8%
Superoxide dismutase (SOD)34172.00:1.002.02:1.001
LCA44411.00:1.0011.72:1.007.92
LACB9201.00:2.221.00:2.124.4
OVAL7351.00:5.001.00:5.510
BSA6241.00:4.001.00:3.5712