Table III Selected putative substrates of SUMO-1 and SUMO-2/3 identified in two IPs that modulate their sumoylation by SUMO1 or SUMO-2/3. Protein expression levels upon hypoxia were not changed or not detected by MS analysis. Further verification of these targets by small scale IPs and WB analysis is marked with an x in a different column
Genes namesProtein namesSUMO1 conjugation in hypoxiaSUMO2/3 conjugation in hypoxiaVerified by WB analysis
ATRXTranscriptional regulator ATRX++x
IRF2BP1Interferon regulatory factor 2-binding protein 1+x
IRF2BP2Interferon regulatory factor 2-binding protein 2+x
NFIL3Nuclear factor interleukin-3-regulated protein+
RREB1Ras-responsive element-binding protein 1+
TMPOLamina-associated polypeptide 2, isoforms beta/gamma; Thymopoietin; Thymopentin+
ZBTB38Zinc finger and BTB domain-containing protein 38++x
ZNF687Zinc finger protein 687+x
BEND3BEN domain-containing protein 3
DBF4Protein DBF4 homolog A
EXOSC10Exosome component 10x
GPC1Glypican-1; Secreted glypican-1
KCTD1BTB/POZ domain-containing protein KCTD1
KCTD15BTB/POZ domain-containing protein KCTD15x
NFRKBNuclear factor related to kappa-B-binding proteinx
NOL8Nucleolar protein 8
PDLIM5PDZ and LIM domain protein 5
TACC2Transforming acidic coiled-coil-containing protein 2
TFAP2ATranscription factor AP-2-alphax
ZFP106Zinc finger protein 106 homologx