Table V

Serine residue hydrogen bonding patterns

The number of observed hydrogen bonds between γ oxygen of a mutated serine and solvent, main chain, and side chain atoms. POS, identity of mutation and position along the chain.

POSOIaTotal HBsbSolvent 20%cSolvent 80%Total solutebMain chain 20%cSide chain 20%Main chain 80%Side chain 80%
G247S*, III9900103010
G382SIV18410114010
G415S*, III, IV13221132010
G448SIV2442081010
G451S*1284084000
G478S*1681091100
G541SIII1720091000
G565S*7210121010
G589SIII, IV11040112110
G598S*18510103010
G601SIII, IV1031192110
G631S*1084191010
G643SIII953092320
G661SIII16510102010
G832SIV12500103010
G844SIII1960071000
G862S*, III1770062010
G871SIII20410102000
G880SIV1532092010
G898SIII13121111010
G901SI1570051010
G913S**7600133211
G964S*852091010
G973SIII1581084010
  • a Lethal OI (Type II) is represented by an asterisk.

  • b Total number of hydrogen bonds represents the total observed hydrogen bonds observed regardless of rate of exchange. The overwhelming majority of observed hydrogen bonds are fast-exchanging hydrogen bonds with solvent.

  • c Solvent 20% and 80% represents the number of observed hydrogen bonds involving the γ oxygen of a mutated serine and any solvent atom that are present in at least 20% and 80% of the snapshots analyzed, respectively. Main chain 20% and 80% and side chain 20% and 80% represent the number of hydrogen bonds involving serine side chains and the peptide main chain and other side chains, respectively.