Table II

Further analysis of selected deletion mutants isolated in the screen

The amount of glycogen and the glycogen synthase activities at 24 and 48 h are normalized to the values obtained with wild-type cells at the same time points. For the qualitative determinations of glycogen on YPD and SC plates, wild-type cells were scored as (+). The activity ratio of glycogen synthase in the wild type strain at the 24-h time point was 0.26 ± 0.02 (n = 6). ND indicates no determination could be made.

Original callGlycogen (iodine) onGlycogen (enzymatic)Glycogen synthaseActivity ratio (24 h)
YPDSC24 h48 h24 h48 h
Protein kinases
KIN1Low+/−+/−0.8821.50.970.11
PTK2Low+/−+0.912.41.30.40.15
RIM15Low0.270.4110.450.07
CTK1High++++ND7.40.43
DBF2High+++2.14.20.820.920.23
NPR1High++1.60.7310.720.04
TOR1High++++1.43.41.110.22
YDL025CHigh+++0.7911.80.520.15
YDR247WHigh+++1.11.70.831.40.28
YNL099CHigh++0.91.910.640.18
YOL045WHigh+++1.13.30.761.80.36
YPL150WHigh++++1.55.81.21.80.18
Protein phosphatases
PIG2Low++/−0.550.261.60.980.09
RTS1High (very)++++++28.51.11.30.17
YCR079WHigh++++1.63.61.31.40.17
WD-40 repeat proteins
YAR003WLow+++0.510.271.10.40.18
YPL247CHigh+++1.40.531.30.870.17
YKL121WHigh+++10.961.20.740.19
YOL087CHigh++0.591.721.60.70.21
YOL138CHigh (very)+++++0.790.521.10.680.55
Vesicular transport and vacuolar function
BST1High (very)+++2.11.3
SEC22High (very)++++2.11.3
VID21High (very)++++++6.56.8220.22
VID22High (very)++2.22.8
VMA10High (very)+++1.85.7
VMA22High (very)++1.23.3
VMA3High+++ND3.5
Inositol metabolism
INO1Low+/−+/−0.350.631.200.04
IPK1Low+/−++0.61.51.21.40.09
Carbohydrate metabolism
RPE1High (very)++++11.10.891.20.12
YHR204WHigh (very)+++1.21.61.71.90.09
Others
ADO1High (very)++++++151.61.70.31
GIS4Low+++/−0.711.31.31.90.09