Further analysis of selected deletion mutants isolated in the screen
The amount of glycogen and the glycogen synthase activities at 24 and 48 h are normalized to the values obtained with wild-type cells at the same time points. For the qualitative determinations of glycogen on YPD and SC plates, wild-type cells were scored as (+). The activity ratio of glycogen synthase in the wild type strain at the 24-h time point was 0.26 ± 0.02 (n = 6). ND indicates no determination could be made.
| Original call | Glycogen (iodine) on | Glycogen (enzymatic) | Glycogen synthase | Activity ratio (24 h) | ||||
|---|---|---|---|---|---|---|---|---|
| YPD | SC | 24 h | 48 h | 24 h | 48 h | |||
| Protein kinases | ||||||||
| KIN1 | Low | +/− | +/− | 0.88 | 2 | 1.5 | 0.97 | 0.11 |
| PTK2 | Low | +/− | + | 0.91 | 2.4 | 1.3 | 0.4 | 0.15 |
| RIM15 | Low | − | − | 0.27 | 0.41 | 1 | 0.45 | 0.07 |
| CTK1 | High | + | +++ | ND | 7.4 | 0.43 | ||
| DBF2 | High | + | ++ | 2.1 | 4.2 | 0.82 | 0.92 | 0.23 |
| NPR1 | High | + | + | 1.6 | 0.73 | 1 | 0.72 | 0.04 |
| TOR1 | High | + | +++ | 1.4 | 3.4 | 1.1 | 1 | 0.22 |
| YDL025C | High | + | ++ | 0.79 | 1 | 1.8 | 0.52 | 0.15 |
| YDR247W | High | + | ++ | 1.1 | 1.7 | 0.83 | 1.4 | 0.28 |
| YNL099C | High | + | + | 0.9 | 1.9 | 1 | 0.64 | 0.18 |
| YOL045W | High | + | ++ | 1.1 | 3.3 | 0.76 | 1.8 | 0.36 |
| YPL150W | High | ++ | ++ | 1.5 | 5.8 | 1.2 | 1.8 | 0.18 |
| Protein phosphatases | ||||||||
| PIG2 | Low | + | +/− | 0.55 | 0.26 | 1.6 | 0.98 | 0.09 |
| RTS1 | High (very) | +++ | +++ | 2 | 8.5 | 1.1 | 1.3 | 0.17 |
| YCR079W | High | + | +++ | 1.6 | 3.6 | 1.3 | 1.4 | 0.17 |
| WD-40 repeat proteins | ||||||||
| YAR003W | Low | + | ++ | 0.51 | 0.27 | 1.1 | 0.4 | 0.18 |
| YPL247C | High | + | ++ | 1.4 | 0.53 | 1.3 | 0.87 | 0.17 |
| YKL121W | High | + | ++ | 1 | 0.96 | 1.2 | 0.74 | 0.19 |
| YOL087C | High | + | + | 0.59 | 1.72 | 1.6 | 0.7 | 0.21 |
| YOL138C | High (very) | ++ | +++ | 0.79 | 0.52 | 1.1 | 0.68 | 0.55 |
| Vesicular transport and vacuolar function | ||||||||
| BST1 | High (very) | + | ++ | 2.1 | 1.3 | |||
| SEC22 | High (very) | ++ | ++ | 2.1 | 1.3 | |||
| VID21 | High (very) | +++ | +++ | 6.5 | 6.8 | 2 | 2 | 0.22 |
| VID22 | High (very) | + | + | 2.2 | 2.8 | |||
| VMA10 | High (very) | ++ | + | 1.8 | 5.7 | |||
| VMA22 | High (very) | + | + | 1.2 | 3.3 | |||
| VMA3 | High | ++ | + | ND | 3.5 | |||
| Inositol metabolism | ||||||||
| INO1 | Low | +/− | +/− | 0.35 | 0.63 | 1.2 | 0 | 0.04 |
| IPK1 | Low | +/− | ++ | 0.6 | 1.5 | 1.2 | 1.4 | 0.09 |
| Carbohydrate metabolism | ||||||||
| RPE1 | High (very) | ++ | ++ | 1 | 1.1 | 0.89 | 1.2 | 0.12 |
| YHR204W | High (very) | ++ | + | 1.2 | 1.6 | 1.7 | 1.9 | 0.09 |
| Others | ||||||||
| ADO1 | High (very) | +++ | +++ | 1 | 5 | 1.6 | 1.7 | 0.31 |
| GIS4 | Low | ++ | +/− | 0.71 | 1.3 | 1.3 | 1.9 | 0.09 |