Table III A priori filters applied in the original TPP analysis workflow [6] to select proteins for hypothesis testing
Rule numberRule
1Both fitted curves for the vehicle and compound treated condition have a coefficient of determination R2 > 0.8, where R2: = 1 − t(xtμc(t))2t(xtx¯)2, with μc(t) being the model prediction for condition c at temperature t, being the mean of all measurements for the protein within a particular condition and replicate, and the summation extending over all temperatures.
2The two curves fitted to the two replicates of the vehicle conditions have a plateau f < 0.3.
3In each biological replicate, the steepest slope of the melting curve in the vehicle and treatment condition needs to be < −0.06 °C−1.